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  2. 疾病研究领域 植物 黄酮类
  3. 芸香科 其他 黄烷醇类
  4. 巴柑檬
  5. Isosakuranetin

Isosakuranetin  (Synonyms: 异野樱素)

目录号: HY-N2131 纯度: 99.88%
COA 产品使用指南

Isosakuranetin 是可以在 Citrus bergamia 的果实中找到的黄烷酮黄酮类化合物。

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Isosakuranetin Chemical Structure

Isosakuranetin Chemical Structure

CAS No. : 480-43-3

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥2145
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1 mg ¥457
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5 mg ¥1950
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10 mg ¥3500
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25 mg ¥6300
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MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Isosakuranetin is a flavanone flavonoid which can be found in the fruit of Citrus bergamia.

体外研究
(In Vitro)

Isosakuranetin stimulates melanin accumulation in a dose-dependent manner. In addition, Isosakuranetin at 30 μM gradually increases the expression of Tyr, TRP1, and TRP2 after 48 and 72 h. The MTT assay shows that Isosakuranetin decreases the number of cells at concentrations greater than 45 μM. Further analysis of trypan blue staining demonstrates that Isosakuranetin does not affect the viability of B16 melanocytes, but shows that Isosakuranetin decreases their proliferation rate. The tyrosinase activity of B16 melanocytes treated with 15 and 30 μM Isosakuranetin for 72 h is significantly increased by 2- and 3.2-fold, respectively, in comparison with that of the control cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Isosakuranetin dose-dependently increases the paw-withdrawal threshold within the dose range of 1.5 to 6 mg/kg. Post hoc analysis reveals that Isosakuranetin increases the paw-withdrawal threshold and statistical significance is seen at times 30 and 40 min for the dose of 3 mg/kg and 30 to 60 min for the dose of 6 mg/kg[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

286.28

Formula

C16H14O5

CAS 号
性状

固体

颜色

White to yellow

中文名称

异野樱素

结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

DMSO 中的溶解度 : 62.5 mg/mL (218.32 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.4931 mL 17.4654 mL 34.9308 mL
5 mM 0.6986 mL 3.4931 mL 6.9862 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

In Vivo:

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂: 10% DMSO    90% Corn Oil

    Solubility: ≥ 20.83 mg/mL (72.76 mM); 澄清溶液

    此方案可获得 ≥ 20.83 mg/mL(饱和度未知)的澄清溶液,此方案实验周期在半个月以上的动物实验酌情使用。

    1 mL 工作液为例,取 100 μL 208.3 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

  • 方案 二

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (7.27 mM); 澄清溶液

    此方案可获得 ≥ 2.08 mg/mL(饱和度未知)的澄清溶液。

    1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

    生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
请输入您的动物体内配方组成:
%
DMSO +
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
连续给药周期超过半月以上,请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: 99.88%

参考文献
Cell Assay
[1]

B16 melanoma cells are cultured in 24-well plates at a density of 1×104 cells/well. After 24 h, the cells are incubated for 72 h in the presence of Isosakuranetin at the indicated concentrations. The culture medium is removed and replaced with 500 μL of fresh culture medium containing 10% sterile filtered MTT. After 3 h, the formazan crystals are dissolved in 500 μL/well isopropanol and absorbance is measured at 570 nm against 630 nm. Inhibition of proliferation (%) is expressed as the percentage of viable treated cells in comparison with control cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Male Sprague-Dawley rats weighing 250 to 300 g are habituated to the temperature, humidity, and lighting (12 h light/dark cycle, lights on at 7: 00 a.m.) controlled vivarium and singly housed for at least 1 week before the studies begin. The rotarod test is used to evaluate the potential motor-impairing effect of Isosakuranetin. For the time course studies, baseline measurement is immediately followed by an injection of Isosakuranetin, and then the paw-withdrawal threshold is measured every 15 min until the drug effect dissipates to a point that the paw withdrawal threshold is not significantly different from the predrug data or until 75 min passes[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4931 mL 17.4654 mL 34.9308 mL 87.3271 mL
5 mM 0.6986 mL 3.4931 mL 6.9862 mL 17.4654 mL
10 mM 0.3493 mL 1.7465 mL 3.4931 mL 8.7327 mL
15 mM 0.2329 mL 1.1644 mL 2.3287 mL 5.8218 mL
20 mM 0.1747 mL 0.8733 mL 1.7465 mL 4.3664 mL
25 mM 0.1397 mL 0.6986 mL 1.3972 mL 3.4931 mL
30 mM 0.1164 mL 0.5822 mL 1.1644 mL 2.9109 mL
40 mM 0.0873 mL 0.4366 mL 0.8733 mL 2.1832 mL
50 mM 0.0699 mL 0.3493 mL 0.6986 mL 1.7465 mL
60 mM 0.0582 mL 0.2911 mL 0.5822 mL 1.4555 mL
80 mM 0.0437 mL 0.2183 mL 0.4366 mL 1.0916 mL
100 mM 0.0349 mL 0.1747 mL 0.3493 mL 0.8733 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Isosakuranetin
目录号:
HY-N2131
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