ASC 抗体

ASC Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 22 kDa, targeting to ASC. It can be used for WB,IHC-F,IHC-P,ICC/IF,ELISA assays with tag free, in the background of Human, Mouse, Rat.

ASC：该基因编码的衔接蛋白由两个蛋白质-蛋白质相互作用结构域组成：N 端 PYRIN-PAAD-DAPIN 结构域 (PYD) 和 C 端半胱天冬酶募集结构域 (CARD)。PYD 和 CARD 结构域是六螺旋束死亡结构域折叠超家族的成员，它通过半胱天冬酶的激活介导炎症和凋亡信号通路中大信号复合物的组装。在正常细胞中，这种蛋白质位于细胞质中；然而，在经历凋亡的细胞中，它在核周边附近形成球状聚集体。已发现该基因的两个转录本变体编码不同的亚型。[由 RefSeq 提供，2008 年 7 月]

Free

Q9ULZ3

Predicted band size: 22 kDa; Observed band size: 22 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102604

Cell Biology

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200 ;ELISA: 1:10,000

• 
  Western blot analysis of extracts from HEK293T (lane 2(20μg) and HEK293T (lane 3(40μg) using ASC(HY-P80548) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using ASC Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80548, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using ASC Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80548, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-F, IHC-P, ICC/IF, ELISA

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human ASC.AA range:10-59.