FGF2 抗体 (YA442)

FGF2 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 31 kDa, targeting to FGF2. It can be used for WB assays with tag free, in the background of Human.

FGF2：该基因编码的蛋白质是成纤维细胞生长因子 (FGF) 家族的成员。FGF 家族成员结合肝素并具有广泛的促有丝分裂和血管生成活性。这种蛋白质与多种生物过程有关，例如肢体和神经系统发育、伤口愈合和肿瘤生长。该基因的 mRNA 包含多个聚腺苷酸化位点，并且可以从非 AUG (CUG) 和 AUG 起始密码子翻译而来，从而产生具有不同特性的五种不同亚型。CUG 启动的亚型位于细胞核中并负责内分泌作用，而 AUG 启动的形式主要是细胞溶质的并且负责该 FGF 的旁分泌和自分泌作用。[由 RefSeq 提供，2008 年 7 月]

Free

P09038

Predicted band size: 31 kDa;Observed band size: 18/22/24 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102657

Cardiovascular

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:500-1:1,000;FC: 1:50-1:100;IP: 1:50;IHC-P: 1:200-1:1,000;

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) , K562 (lane 3(20μg) , and HEK293T (lane 4(20μg)using FGF2 (HY-P80671) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80671, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Flow cytometric analysis of 1X10^6 Jurkat cells labeling FGF2 Antibody(HY-P80671, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

WB, IP, FC, IHC-P

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide within Human FGF2 aa 121-170 / 288.