2. 抗体
  3. 一抗
  4. 单克隆抗体
  5. Histone H3 (tri methyl K9) 抗体 (YA734)

Histone H3 (tri methyl K9) 抗体 (YA734)

目录号: HY-P80176
COA 抗体使用指南 技术支持

Histone H3 (tri methyl K9) Antibody 是一个非偶联、分子量约 15 kDa、小鼠来源的抗 Histone H3 (tri methyl K9) 单克隆抗体。它可用于人、小鼠、大鼠背景下 WB、ICC、IHC-P 实验，且不带标记。

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规格 价格 是否有货 数量
10 μL ￥470 In-stock
50 μL ￥1220 In-stock
100 μL ￥2000 In-stock
250 μL   询价  

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Top Publications Citing Use of Products

【WB: 蛋白质免疫印迹; IHC-P: 石蜡切片样本的免疫组织化学; IHC-F: 冰冻切片样本的免疫组织化学; ICC/IF: 细胞免疫荧光; IF-Tissue: 组织免疫荧光; mIHC: 多重荧光免疫组化; IP: 免疫沉淀; ChIP: 染色质免疫沉淀; FC: 流式细胞术; ELISA: 酶联免疫吸附试验】

  • 生物活性

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描述

Histone H3 (tri methyl K9) Antibody is a non-conjugated and Mouse origined monoclonal antibody about 15 kDa, targeting to Histone H3 (tri methyl K9). It can be used for WB,ICC,IHC-P assays with tag free, in the background of Human, Mouse, Rat.
研究背景

组蛋白 H3 是核小体的基本组成部分，在将 DNA 包裹和压缩到染色质的复杂过程中发挥着关键作用，这反过来又限制了 DNA 进入需要 DNA 作为模板的细胞机器。这种组蛋白及其对应物在关键细胞功能中发挥着关键作用，包括转录调节、DNA 修复、DNA 复制和染色体稳定性的维持。DNA 可及性的调节涉及翻译后修饰（统称为组蛋白密码）的复杂相互作用，以及核小体的动态重塑。核小体本身包含组蛋白八聚体，由 H2A、H2B、H3 和 H4 各两个分子组成，组装成一个 H3-H4 异四聚体和两个 H2A-H2B 异二聚体。这种八聚体有效地包裹了大约 147 个碱基对的 DNA，证明了它在组织染色质结构和促进关键基因组过程中不可或缺的作用。此外，组蛋白 H3 与各种细胞成分（例如 TONSL、CHAF1A、CHAF1B、MCM2 和 DNAJC9）相互作用，进一步促进其多方面功能。
标签

Free
基因 ID
蛋白数据库
中文名
Histone H3 (tri methyl K9) 抗体
同用名
H3K9me3; H3 histone family member E pseudogene; H3 histone family, memberA; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; HistoneH3/l; H3K9me2; HistoneH3(trimethylK9)
分子量

Predicted band size: 15 kDa; Observed band size: 15 kDa
纯度

Protein G affinity purified.
亚细胞定位

Nucleus.
偶联

Non-conjugated
修饰

Methylated
RRID
研究领域

Epigenetics and Nuclear Signaling
产品类别

Primary Antibody; Mouse Monoclonal Antibody
克隆性

Monoclonal
宿主

Mouse
反应物种

Human, Mouse, Rat
推荐稀释比例

WB: 1:5000-1:10000 ;ICC: 1:500-1:1000 ;IHC-P: 1:200

  • Western blot analysis of extracts from HCT116(lane 2(20μg) and HCT116(lane 3(40μg) using Histone H3 (tri methyl K9) (HY-P80176) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80176, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
应用

WB, ICC/IF, IHC-P
性状

液体
组分

Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
同型

IgG1
敏感性

Endogenous
免疫原

Synthetic peptide corresponding to Human Histone H3.The exact sequence is proprietary to MCE.
数据库

Entrez Gene: 8350 Human ; 319152 Mouse ; 291159 Rat

SwissProt: P68431 Human ; P68433 Mouse ; Q6LED0 Rat

OMIM: 137800 Human

文件资料

COA (193 KB)

抗体使用指南 (1083 KB)

Histone H3 (tri methyl K9) Antibody (YA734) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称：
Histone H3 (tri methyl K9) Antibody (YA734)
目录号：
HY-P80176
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