Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) 抗体

Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 84 kDa, targeting to Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199). It can be used for WB,IHC-F,IHC-P,ICC/IF,ELISA assays with tag free, in the background of Human, Mouse, Rat.

PI3 Kinase p85/p55：磷脂酰肌醇 3-激酶在 3-素数位置磷酸化磷脂酰肌醇的肌醇环。该酶包含一个 110 kD 的催化亚基和一个 85、55 或 50 kD 的调节亚基。该基因编码 85 kD 调节亚基。磷脂酰肌醇 3-激酶在胰岛素的代谢作用中起着重要作用，该基因的突变与胰岛素抵抗有关。该基因的可变剪接导致编码不同亚型的四种转录变体。[由 RefSeq 提供，2011 年 6 月]

Free

P27986

Predicted band size: 84 kDa；Observed band size: 55,85 kDa

affinity purified

Non-conjugated

Phosphorylated

AB_3102229

Signal Transduction

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200 ;ELISA: 1:10,000

• 
  Western blot analysis of extracts from HEK293(lane 2(40μg) and HEK293(lane 3(80μg) using Phospho-PI3 Kinase p85/p55 (Tyr467/Tyr199) (HY-P80790) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of NIH3T3 cells labeling Phospho-PI3 Kinase p85 with Phospho-PI3 Kinase p85 antibody (HY-P80846) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-PI3 Kinase p85 antibody (HY-P80970) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of NIH3T3 cells labeling Phospho-PI3 Kinase p85 with Phospho-PI3 Kinase p85 antibody (HY-P80846) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-PI3 Kinase p85 antibody (HY-P80846) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, IHC-F, IHC-P, ICC/IF, ELISA

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Tyr467/199 of Human PI3-kinase p85-alpha/gamma.AA range:436-485.