2. JAK/STAT Signaling Protein Tyrosine Kinase/RTK Cell Cycle/DNA Damage PI3K/Akt/mTOR
  3. EGFR PERK Akt
  4. GC1118

GC1118  (Synonyms: GC-1118A)

目录号: HY-P991571
技术支持

GC1118 (GC-1118A) 是一种全人源的抗 EGFR 单克隆抗体，对 EGFR 的 KD 值为 0.16 nM。GC1118 对高亲和力和低亲和力 EGFR 配体诱导的信号传导均显示出强效抑制作用。GC1118 在 KRAS 野生型和 KRAS 突变型细胞中均表现出强大的抗增殖活性。GC1118 能够穿过血脑屏障 (BBB) 和血瘤屏障 (BTB) 到达肿瘤部位，并且在多种小鼠异种移植瘤模型中显示出卓越的抗肿瘤效果。GC1118 可以用于癌症的研究，如结直肠癌[1][2][3][4]。

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GC1118 Chemical Structure

GC1118 Chemical Structure

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GC1118 相关抗体

Top Publications Citing Use of Products

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EGFR/ErbB1/HER1 ErbB2/HER2 ErbB3/HER3 ErbB4/HER4

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

GC1118 (GC-1118A) is a fully human anti-EGFR monoclonal antibody with binding affinity of 0.16 nM (KD) to EGFR. GC1118 displays potent inhibitory effects on high- and low-affinity EGFR ligand-induced signaling. GC1118 shows potent anti proliferative activity in KRAS wild-type and KRAS mutant cells. GC1118 can reach the tumor by crossing both BBB (blood-brain barrier) and BTB (brain-tumor barrier) and shows superior anti-tumor effects in various mice xenograft models. GC1118 can be used for the researches of cancer, such as colorectal cancer[1][2][3][4].

IC50 & Target

EGFR
体外研究
(In Vitro)

GC1118 (GC-1118A) (1 μg/mL, 24-120 h) 在 KRAS 野生型和 KRAS 突变型细胞中显示出强大的抗增殖活性[1][4]。

GC1118 (100 µM, 6 days) 抑制 G096 和 G022 的原代肿瘤细胞的生长[2]。

GC1118 (0.1-50 μg/mL, 2 h) 可以阻断 HCT8 细胞中 EGFR 高亲和力和低亲和力配体诱导的 EGFR 信号传导[3]。

GC1118 (0.005-100 μg/mL, 3 days) 抑制 HCT8 细胞中高亲和力和低亲和力配体诱导的细胞增殖[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

GC1118 相关抗体:

Cell Proliferation Assay[1]

Cell Line: SNU-1, SNU-5, SNU-16, SNU-216, SNU-484, SNU-601, SNU-620, SNU-638, SNU-668, SNU-719, AGS, MKN-45 and NCI-N87 cells
Concentration: 0, 0.001, 0.01, 0.1, 1, 10, 100 and 1000 µg/ mL
Incubation Time: 72 h
Result: Suppressed cell growth, particularly in the MKN-45 cell line.

Cell Proliferation Assay[1]

Cell Line: SNU-484, SNU-601, SNU-719 and MKN-45 cells
Concentration: 1 µg/mL
Incubation Time: 24, 72, 120 h
Result: Showed more obviously anti-growth inhibitory effect in KRAS wild-type cell lines, SNU-719 and MKN-45, but also in KRAS mutant SNU-601 cells.

Western Blot Analysis[3]

Cell Line: HCT8 cells
Concentration: 0.1, 0.5, 5 and 50 μg/mL
Incubation Time: 2 h, stimulated with EGFR ligands (250 ng/mL EGF, HB-EGF, BTC, and TGF-α; 300 ng/mL AREG; 500 ng/mL EREG)
Result: Inhibited the high- and low-affinity ligand-induced Y1068 phosphorylation, Akt and Erk levels.
体内研究
(In Vivo)

GC1118 (GC-1118A) (1 mg/kg；腹腔注射；一周 2 次，持续 5 周) 可显著抑制 AGS 异种移植瘤小鼠模型中的肿瘤的生长[1]。

GC1118 (50 mg/kg；腹腔注射；一周 2 次) 在患者来源的 GBM 异种移植瘤模型中显示出卓越的抗肿瘤活性[2]。

GC1118 (1 mg/mouse；腹腔注射；一周 2 次，持续 5 周) 可显著抑制 HCT8、Lovo、HCT15、LS174T、LS513 和 SW48 异种移植瘤小鼠模型中的肿瘤生长[3]。

GC1118 (1 mg/mouse；腹腔注射；一周 2 次，持续 52 天) 在患者来源的 CRC-024T 异种移植瘤小鼠模型中展现出中度的抗肿瘤作用[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: AGS xenograft mice models (athymic nude mice, female, 6 weeks, tumor volume of 200 mm3)[1]
Dosage: 1 mg/kg
Administration: Intraperitoneally injection, twice a week for 5 weeks
Result: Showed a significant suppression of the tumor growth.
Animal Model: Patient-derived GBM xenograft models (BALB/c nude mice, female, 6-8 weeks, tumor volume of 150–200 mm3)[2]
Dosage: 50 mg/kg
Administration: Intraperitoneal injection; twice a week until euthanasia
Result: Exerted anti-tumor effects in eight PDXs (G022, G677, B802, G608, G542, G096, G698, and G500).
Showed significantly better survival outcome than the control group.
Showed a significant amount accumulated in the tumor core.
Significantly increased tumor cell apoptosis and reduced microvascular density (MVD).
Animal Model: HCT8, Lovo, HCT15, LS174T, LS513, and SW48 xenograft mice models (athymic nude mice, 7-8 weeks, tumor volume of 200 mm3)[3]
Dosage: 1 mg/mouse
Administration: Intraperitoneally injection, twice a week for 5 weeks
Result: Significantly suppressed the tumor growth.
Animal Model: CRC-024T patient-derived xenograft mice models (BALB/c-nude mice, female, 6-8 weeks, tumor volume of 200-250 mm3)[4]
Dosage: 1 mg/mouse
Administration: Intraperitoneally injection, twice a week for 52 days
Result: Had moderate antitumor effects and no reduction in body weight.
Downregulated the levels of AKT and ERK1/2.
基因 ID

1956  [NCBI]

Accession

P00533-1
靶点

EGFR
应用

ELISA, FACS, Functional assay
偶联物

Unconjugated
复溶方法

The product can be reconstituted/diluted with sterile PBS or saline.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

GC1118 相关分类

  • 摩尔计算器

  • 稀释计算器

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GC1118GC-1118AGC 1118GC-1118EGFRPERKAktEpidermal growth factor receptorErbB-1HER1Protein kinase R-like endoplasmic reticulum kinasePKR-like endoplasmic reticulum kinasePKBProtein kinase BCancerERKMKN-45 cellsHCT8 cellsPDXsInhibitorinhibitorinhibit

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