1. Academic Validation
  2. SLC26A9 promotes colorectal tumorigenesis by modulating Wnt/β-catenin signaling

SLC26A9 promotes colorectal tumorigenesis by modulating Wnt/β-catenin signaling

  • Cell Death Discov. 2024 Mar 9;10(1):123. doi: 10.1038/s41420-024-01888-6.
Minglin Zhang # 1 Zhiyuan Ma # 1 Zhiqiang Yi # 1 Hu Wang 2 Jiaxing Zhu 1 Guorong Wen 1 Hai Jin 1 Jiaxing An 1 Zilin Deng 1 Biguang Tuo 3 Taolang Li 4 Xuemei Liu 5
Affiliations

Affiliations

  • 1 Department of Gastroenterology, Digestive Disease Hospital, Affiliated Hospital of Zunyi Medical University, Zunyi, China.
  • 2 Department of General Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, China.
  • 3 Department of Gastroenterology, Digestive Disease Hospital, Affiliated Hospital of Zunyi Medical University, Zunyi, China. tuobiguang@aliyun.com.
  • 4 Department of General Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, China. 0078029@sina.com.
  • 5 Department of Gastroenterology, Digestive Disease Hospital, Affiliated Hospital of Zunyi Medical University, Zunyi, China. onlyoneliuxuemei@163.com.
  • # Contributed equally.
Abstract

Solute carrier family 26 member 9 (SLC26A9) is a member of the Slc26a family of multifunctional anion transporters that functions as a Cl- channel in parietal cells during acid secretion. We explored the role of SLC26A9 in colorectal Cancer (CRC) and its related mechanisms through clinical samples from CRC patients, CRC cell lines and mouse models. We observed that SLC26A9 was expressed at low levels in the cytoplasm of adjacent tissues, polyps and adenomas but was significantly increased in colorectal adenocarcinoma. Moreover, increased levels of SLC26A9 were associated with a high risk of disease and poor prognosis. In addition, downregulation of SLC26A9 in CRC cells induced cell cycle arrest and Apoptosis but inhibited cell proliferation and xenograft tumor growth both in vitro and in vivo. Mechanistic analysis revealed that SLC26A9 was colocalized with β-catenin in the nucleus of CRC cells. The translocation of these two proteins from the cytoplasm to the nucleus reflected the activation of Wnt/β-catenin signaling, and promoted the transcription of downstream target proteins, including CyclinD1, c-Myc and Snail, but inhibited the expression of cytochrome C (Cyt-c), cleaved Caspase9, cleaved Caspase3 and apoptosis-inducing factor (AIF). CRC is accompanied by alteration of epithelial mesenchymal transition (EMT) markers. Meanwhile, further studies showed that in SW48 cells, overexpressing SLC26A9 was cocultured with the β-catenin Inhibitor XAV-939, β-catenin was downregulated, and EMT was reversed. Our study demonstrated SLC26A9 may be responsible for alterations in the proliferative ability and aggressive potential of CRC by regulating the Wnt/β-catenin signaling pathway.

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