SKF-96365 hydrochloride

目录号: HY-100001 纯度: 99.71%: Data Sheet SDS COA 产品使用指南
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技术支持

SKF-96365 hydrochloride 是一种有效的 TRP channel 阻滞剂和 SOCE (store-operated Ca²⁺ entry) 抑制剂。SKF-96365 hydrochloride 可显著抑制豚鼠离体心脏 hERG、hKCNQ1/hKCNE1、hKir2.1 和 hKv4.3 电流，显著延长 QTc 间期。SKF-96365 hydrochloride 通过诱导结直肠癌细胞的细胞周期阻滞和凋亡 (apoptosis) 而显示出强大的抗肿瘤活性。

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SKF-96365 hydrochloride Chemical Structure

CAS No. : 130495-35-1

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可免费申领三个不同产品的试用装。

3. 试用装只面向终端客户。

规格	价格	是否有货
10 mM * 1 mL in DMSO	￥715	In-stock
5 mg	￥623	In-stock
10 mg	￥860	In-stock
25 mg	￥1892	In-stock
50 mg	￥3500	In-stock
100 mg	￥5413	In-stock
200 mg	现货	询价
500 mg		询价
1 g		询价

or 大包装询价

* Please select Quantity before adding items.

Customer Review

MCE 顾客使用本产品发表的 22 篇科研文献

SKF-96365 hydrochloride 相关产品

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TRPC TRPM TRPV TRPA TRPML

生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

SKF-96365 hydrochloride is a potent TRP channel blocker and a store-operated Ca²⁺ entry (SOCE) inhibitor. SKF-96365 hydrochloride significantly inhibits hERG, hKCNQ1/hKCNE1, hKir2.1 and hKv4.3 current, and significantly prolongs the QTc interval in isolated guinea pig hearts. SKF-96365 hydrochloride exhibits potent anti-neoplastic activity by inducing cell-cycle arrest and apoptosis in colorectal cancer cells^[1]^[2].

体外研究
(In Vitro)

SKF-96365 通过诱导结直肠癌细胞的细胞周期停滞和凋亡，展现出强大的抗肿瘤活性。SKF-96365 以浓度依赖的方式抑制 hERG 电流^[1]。SKF-96365 可诱导细胞保护性自噬，通过阻止细胞色素 c (cyt c) 从线粒体释放到细胞质中来延缓细胞凋亡。从机制上讲，使用 SKF-96365 进行处理会抑制钙/钙调蛋白依赖性蛋白激酶 IIγ (CaMKIIγ)/AKT 信号级联反应。过表达 CaMKIIγ 或 AKT 可消除 SKF-96365 对癌细胞的作用，这表明 CaMKIIγ/AKT 信号通路在 SKF-96365 诱导的生物学效应中起着关键作用^[2]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

SKF-96365 可在体内抑制 CRC 细胞的生长。SKF-96365 处理小鼠后，p-CaMKII 和 p-AKT水平降低，LC3-II、cleaved PARP、caspase-3 和 caspase-9 水平升高^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

402.91

Formula

C₂₂H₂₇ClN₂O₃

CAS 号

130495-35-1

性状

固体

颜色

White to light yellow

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

细胞实验：

H₂O 中的溶解度 : 100 mg/mL (248.19 mM; 超声助溶; DMSO can inactivate SKF-96365 hydrochloride's activity)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.4819 mL	12.4097 mL	24.8194 mL
5 mM	0.4964 mL	2.4819 mL	4.9639 mL
10 mM	0.2482 mL	1.2410 mL	2.4819 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： Saline
Solubility: 100 mg/mL (248.19 mM); 澄清溶液; 超声助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

该产品水溶性佳，请具体参考实测水 / PBS / Saline 中的溶解度数据。

您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。

纯度 & 产品资料

纯度: 99.71%

选择批次：

Data Sheet (637 KB) SDS (393 KB)

COA (287 KB) HNMR (263 KB) LCMS (90 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Liu H, et al. SKF-96365 blocks human ether-à-go-go-related gene potassium channels stably expressed in HEK 293 cells. Pharmacological Research. Pharmacol Res, 2016 Feb, 104:61-9. [Content Brief]

[2]. Jing Z, et al. SKF-96365 activates cytoprotective autophagy to delay apoptosis in colorectal cancer cells through inhibition of the calcium/CaMKIIγ/AKT-mediated pathway. Cancer Lett, 2016 Mar 28, 372(2):226-38. [Content Brief]

Animal Administration ^[2]	Mice: Five to six-week-old female athymic BALB/c mice are inoculated into the right oxter with HCT116 cells. When the diameter of the subcutaneous tumor reaches approximately 0.5 cm, animals are randomLy assigned to the vehicle, SKF-96365 alone, HCQ alone or SKF-96365+HCQ. SKF-96365 is applied (20 mg/kg) and HCQ is applied (60 mg/kg) daily for 14 successive days by i.p. injection. Tumor sizes and volume are determined. Eight mice are included in each group. Mice are sacrificed 24 h after the last treatment. The tumors are weighed and processed for western blot analysis or paraffin embedding^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Liu H, et al. SKF-96365 blocks human ether-à-go-go-related gene potassium channels stably expressed in HEK 293 cells. Pharmacological Research. Pharmacol Res, 2016 Feb, 104:61-9. [Content Brief] [2]. Jing Z, et al. SKF-96365 activates cytoprotective autophagy to delay apoptosis in colorectal cancer cells through inhibition of the calcium/CaMKIIγ/AKT-mediated pathway. Cancer Lett, 2016 Mar 28, 372(2):226-38. [Content Brief]

Animal Administration
^[2]

Mice: Five to six-week-old female athymic BALB/c mice are inoculated into the right oxter with HCT116 cells. When the diameter of the subcutaneous tumor reaches approximately 0.5 cm, animals are randomLy assigned to the vehicle, SKF-96365 alone, HCQ alone or SKF-96365+HCQ. SKF-96365 is applied (20 mg/kg) and HCQ is applied (60 mg/kg) daily for 14 successive days by i.p. injection. Tumor sizes and volume are determined. Eight mice are included in each group. Mice are sacrificed 24 h after the last treatment. The tumors are weighed and processed for western blot analysis or paraffin embedding^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Liu H, et al. SKF-96365 blocks human ether-à-go-go-related gene potassium channels stably expressed in HEK 293 cells. Pharmacological Research. Pharmacol Res, 2016 Feb, 104:61-9. [Content Brief]

[2]. Jing Z, et al. SKF-96365 activates cytoprotective autophagy to delay apoptosis in colorectal cancer cells through inhibition of the calcium/CaMKIIγ/AKT-mediated pathway. Cancer Lett, 2016 Mar 28, 372(2):226-38. [Content Brief]

SKF-96365 hydrochloride 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.4819 mL	12.4097 mL	24.8194 mL	62.0486 mL
	5 mM	0.4964 mL	2.4819 mL	4.9639 mL	12.4097 mL
	10 mM	0.2482 mL	1.2410 mL	2.4819 mL	6.2049 mL
	15 mM	0.1655 mL	0.8273 mL	1.6546 mL	4.1366 mL
	20 mM	0.1241 mL	0.6205 mL	1.2410 mL	3.1024 mL
	25 mM	0.0993 mL	0.4964 mL	0.9928 mL	2.4819 mL
	30 mM	0.0827 mL	0.4137 mL	0.8273 mL	2.0683 mL
	40 mM	0.0620 mL	0.3102 mL	0.6205 mL	1.5512 mL
	50 mM	0.0496 mL	0.2482 mL	0.4964 mL	1.2410 mL
	60 mM	0.0414 mL	0.2068 mL	0.4137 mL	1.0341 mL
	80 mM	0.0310 mL	0.1551 mL	0.3102 mL	0.7756 mL
	100 mM	0.0248 mL	0.1241 mL	0.2482 mL	0.6205 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

SKF-96365 hydrochloride

Customer Review

MCE 顾客使用本产品发表的 22 篇科研文献