2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species
  4. NucPE1

NucPE1  (Synonyms: Nuclear Peroxy Emerald 1)

目录号: HY-101859 纯度: ≥98.0%
COA 产品使用指南

摩尔计算器

NucPE1 (Nuclear Peroxy Emerald 1) 是核定位荧光过氧化氢探针, 其特异性定位于细胞核，没有附加的靶向部分。

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NucPE1 Chemical Structure

NucPE1 Chemical Structure

CAS No. : 1404091-23-1

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
1 mg ￥1500
In-stock
5 mg ￥4500
In-stock
10 mg ￥7500
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

NucPE1 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

NucPE1 (Nuclear Peroxy Emerald 1) is a nuclear-localized fluorescent hydrogen peroxide that is specifically localized to cellular nuclei without appended targeting moieties.

体外研究
(In Vitro)

NucPE1 features two major visible region absorptions (λabs=468 nm, ε=27,300 M-1cm-1; λabs=490 nm, ε=26,000 M-1cm-1) and a weak emission (λem=530 nm, Φ=0.117). Reaction of NucPE1 with H2O2 triggers a fluorescence increase upon its conversion to fluorophore NucPE1, which possesses one major absorption band at 505 nm (ε=19,100 M-1cm-1) with enhanced emission (λem=530 nm, Φ=0.626). NucPE1 selectively accumulates in the nuclei of a variety of mammalian cell lines as well as in whole model organisms like C. clegans, where it can respond to subcellular changes in H2O2 fluxes[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

NucPE1 相关抗体:
体内研究
(In Vivo)

NucPE1 maintains the ability to selectively target nuclei in vivo. NucPE1 imaging reveals a reduction in nuclear H2O2 levels in worms overexpressing sir-2.1 compared to wildtype congeners, supporting a link between this longevity-promoting sirtuin protein and enhanced regulation of nuclear ROS pools[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

483.36

Formula

C29H30BNO5
CAS 号
性状

固体

颜色

White to off-white

Emission (Em)

530
Excitation (Ex)

490
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

溶解性数据
In Vitro: 

DMSO 中的溶解度 : 25 mg/mL (51.72 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.0689 mL 10.3443 mL 20.6885 mL
5 mM 0.4138 mL 2.0689 mL 4.1377 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

In Vivo:

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.17 mM); 澄清溶液

    此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

    生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
请输入您的动物体内配方组成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
计算结果
工作液所需浓度 : mg/mL
储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
连续给药周期超过半月以上，请谨慎选择该方案。
请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
纯度 & 产品资料

纯度: ≥98.0%

COA (269 KB) HNMR (301 KB)

产品使用指南 (1538 KB)
参考文献
Cell Assay
[1][2]

Excitation of NucPE1-loaded cells at 514 nm is carried out with an Ar laser and emission is collected using a META detector between 522–554 nm. Excitation of Hoechst 33342 is carried out using a MaiTai two-photon laser at 780-nm pulses and emission is collected between 436–501 nm. All images in an experiment are collected simultaneously using identical microscope settings. Image analysis is performed in Image J. The background fluorescence is measured in a data set by selecting an ROI outside of the cells. This background number is then used to set the threshold of the image and thereby select for NucPE1 signal. The mean fluorescence intensity of this signal is then measured and utilized as the fluorescent signal for that particular image. The same threshold settings are utilized for all images in an experiment[1]. The measurement of nuclear H2O2 is achieved using NucPE1. The cells are incubated for 45 min at 10 μM NucPE1 in the dark. The incubation is followed by ishing and analysis by flow cytometry as explained above. Mitochondrial ROS are measured using MitoSOX probe. The cells are incubated with 5 μM MitoSOX for 15 min in the dark. The incubation is followed by ishing and analysis by flow cytometry[2]. Live imaging of the NucPE1 probe is carried out using excitation at 488 nm with an argon laser, and emission is collected using a META detector at about 520 nm. The Hoechst dye is incubated together with NucPE1. When multiple staining of NucPE1 and PO1 is performed, the multitracking mode of scanning is applied for acquisition of the images[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

NucPE1 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0689 mL 10.3443 mL 20.6885 mL 51.7213 mL
5 mM 0.4138 mL 2.0689 mL 4.1377 mL 10.3443 mL
10 mM 0.2069 mL 1.0344 mL 2.0689 mL 5.1721 mL
15 mM 0.1379 mL 0.6896 mL 1.3792 mL 3.4481 mL
20 mM 0.1034 mL 0.5172 mL 1.0344 mL 2.5861 mL
25 mM 0.0828 mL 0.4138 mL 0.8275 mL 2.0689 mL
30 mM 0.0690 mL 0.3448 mL 0.6896 mL 1.7240 mL
40 mM 0.0517 mL 0.2586 mL 0.5172 mL 1.2930 mL
50 mM 0.0414 mL 0.2069 mL 0.4138 mL 1.0344 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NucPE11404091-23-1Nuclear Peroxy Emerald 1NucPE 1NucPE-1Nuclear Peroxy Emerald1Nuclear Peroxy Emerald-1Reactive Oxygen SpeciesInhibitorinhibitorinhibit

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