APG5L 抗体

APG5L Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 32 kDa, targeting to APG5L. It can be used for WB,ICC/IF,IP assays with tag free, in the background of Human, Mouse, Rat.

APG5L：该基因编码的蛋白质与自噬蛋白 12 结合，在泛素样结合系统中起 E1 样激活酶的作用。编码的蛋白质参与多种细胞过程，包括自噬囊泡形成、氧化损伤后线粒体质量控制、先天抗病毒免疫反应的负调节、淋巴细胞发育和增殖、MHC II 抗原呈递、脂肪细胞分化和细胞凋亡。已发现该基因的几种转录变体编码不同的蛋白质亚型。[由 RefSeq 提供，2015 年 9 月]

Free

9474

Predicted band size: 32 kDa；Observed band size: 55 kDa

affinity purified

Non-conjugated

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Rabbit

Human, Mouse, Rat

Q9H1Y0

WB: 1:500-1:1,000 ;IF: 1:50-1:200 ;IP: 1:20

• 
  Western blot analysis of extracts from Raji(lane 2(20μg) ), Hela(lane 3(20μg) ) ,RAW264.7(lane 4(20μg) ) and NIH3T3(lane 5(20μg)) using APG5L(HY-P80545) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80545, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB, ICC/IF, IP

液体

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Synthetic peptide corresponding to Human APG5L/ATG5.The exact sequence is proprietary to MCE.