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  4. Histone H3 (acetyl K14) 抗体 (YA375)

Histone H3 (acetyl K14) 抗体 (YA375)

目录号: HY-P80167
COA 抗体使用指南 技术支持

Histone H3 (acetyl K14) Antibody 是一个非偶联、分子量约 15 kDa、兔源、抗 Histone H3 (acetyl K14) 单克隆抗体。它可用于人、小鼠、大鼠背景下 WB、ICC/IF、IHC-P、IP、SNAP-ChIP、CUT&Tag-seq 实验,且不带标记。

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规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

【WB: 蛋白质免疫印迹; IHC-P: 石蜡切片样本的免疫组织化学; IHC-F: 冰冻切片样本的免疫组织化学; ICC/IF: 细胞免疫荧光; IF-Tissue: 组织免疫荧光; mIHC: 多重荧光免疫组化; IP: 免疫沉淀; ChIP: 染色质免疫沉淀; FC: 流式细胞术; ELISA: 酶联免疫吸附试验】

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描述

Histone H3 (acetyl K14) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 15 kDa, targeting to Histone H3 (acetyl K14). It can be used for WB,ICC/IF,IHC-P,IP,SNAP-ChIP,CUT&Tag-seq assays with tag free, in the background of Human, Mouse, Rat.

标签

Free

基因 ID
蛋白数据库
中文名
Histone H3 (acetyl K14) 抗体
分子量

Predicted band size: 15 kDa; Observed band size: 15 kDa

纯度

Protein A affinity purified.

亚细胞定位

Nucleus. Chromosome.

偶联

Non-conjugated

修饰

Acetylated

RRID
研究领域

Epigenetics and Nuclear Signaling

产品类别

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

克隆性

Recombinant, Monoclonal

宿主

Rabbit

反应物种

Human, Mouse, Rat

推荐稀释比例

WB: 1:500-1:2,000 ;ICC/IF: 1:200-1:1,000 ;IHC-P: 1:50-1:200 ;SNAP-ChIP: 1:100 ;IP: Use at an assay dependent concentration.

  • Western blot analysis of extracts from 293T (lane 1) and Hela (lane 2) and Ramos (lane 3) and Jurkat (lane 4) using Histone H3 (acetyl K14) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
应用

WB, ICC/IF, IHC-P, IP, ChIP

性状

液体

组分

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

同型

IgG

敏感性

Endogenous

免疫原

Synthetic peptide corresponding to Human Histone H3.AA range:1-50.

数据库
文件资料

Histone H3 (acetyl K14) Antibody (YA375) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Histone H3 (acetyl K14) Antibody (YA375)
目录号:
HY-P80167
需求量: