CellTracker Orange CMRA Dye 

CellTracker Orange CMRA Dye is a fluorescent dye. CellTracker Orange CMRA Dye can be used for cell imaging and monitoring the movement and location of cells^[1].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Cell viability assay^[1]:
1. Adds 5 μM CellTracker Orange CMRA Dye to each sample at 27-33℃ for 30 min to label sample.
2. Centrifuges cells 1000 g for 10 min, resuspends cell pellet in pre-warmed 10% iFCS RPMI (3*10⁷cells/Ml).
3. Incubates cells at 27-33℃ for 30 min.
4. Centrifuges cells 1000 g for 10 min and wash with pre-warmed PBS without Ca²⁺ and Mg²⁺.
5. Centrifuges cells 1000 g for 10 min and cells resuspends in pre-warmed 10% iFCS RPMI.
6. Measure fluorescence intensity (Ex=548 nm, Em=576 nm).

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

550.43

C₃₀H₂₅Cl₂NO₅

576

548

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Dagley MJ, et al. High-content assay for measuring intracellular growth of Leishmania in human macrophages. Assay Drug Dev Technol. 2015 Sep;13(7):389-401.  [Content Brief]