2. Protein Tyrosine Kinase/RTK Apoptosis
  3. IGF-1R Apoptosis
  4. Dalotuzumab

Dalotuzumab  (Synonyms: MK-0646; h7C10)

目录号: HY-P99284 纯度: 97.43%
COA

摩尔计算器

Dalotuzumab (MK-0646) 是一种靶向 IGF-1R 的重组人源化单克隆抗体 (IgG1 型)。Dalotuzumab 通过抑制 IGF-1 和 IGF-2 介导的肿瘤细胞增殖、IGF-1R 自体磷酸化和 Akt 磷酸化而发挥作用。Dalotuzumab 还可诱导细胞凋亡和周期停滞。Dalotuzumab 与其他抗癌活性分子（如他汀类活性分子）共同使用，可增强 Dalotuzumab 的体外和体内抗肿瘤活性。

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Dalotuzumab Chemical Structure

Dalotuzumab Chemical Structure

CAS No. : 1005389-60-5

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1 mg ￥3000
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5 mg ￥7800
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Customer Review

Dalotuzumab 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Dalotuzumab (MK-0646) is a recombinant humanized monoclonal antibody (IgG1 type) targeting IGF-1R. Dalotuzumab acts by inhibiting IGF-1- and IGF-2-mediated tumor cell proliferation, IGF-1R autophosphorylation, and Akt phosphorylation. Dalotuzumab also induces apoptosis and cycle arrest. Dalotuzumab in combination with other anticancer agents such as statins can enhance the antitumor activity of Dalotuzumab in vitro and in vivo[1][2][3].

同型

Human IgG1 kappa
推荐同型对照抗体
种属

Humanized
体外研究
(In Vitro)

Dalotuzumab (h7C10; 33 nM; 24 h) 抑制 IGF-1 和 IGF-2 诱导的 MCF7 雌激素依赖性乳腺癌细胞增殖，IC50 值分别为 4.2 和 3.1 nM[1]。
Dalotuzumab (33 nM; 24 h) 在 IGF-1 诱导的 MCF7 细胞中，诱导周期停滞，并抑制 IGF-IR 和 IRS-1 的自磷酸化[1]。
Dalotuzumab 使 NK 细胞介导的 MCF7 和 A459 细胞的裂解率分别提高了 26% 和 25%[1]。
Dalotuzumab (MK-0646; 10 µg/mL; 24, 48 h) 消除 IGF1 在子宫内膜癌细胞中的抗凋亡作用[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Dalotuzumab 相关抗体:

Cell Proliferation Assay[1]

Cell Line: MCF7 cells (IGF-1 and IGF-2-induced)
Concentration: 33 nM
Incubation Time: 24 h
Result: Showed anti-proliferation activity to IGF-1- and IGF-2-induced MCF7, with IC50 values of 4.2 and 3.1 nM, respectively.

Cell Cycle Analysis[1]

Cell Line: MCF7 cells (IGF-1-induced)
Concentration: 33 nM
Incubation Time: 24 h
Result: Prevented cell cycle progression from the G1 to S and G2/M phases.

Apoptosis Analysis[2]

Cell Line: ECC-1 and USPC-1 cells (IGF-1-induced)
Concentration: 10 µg/mL
Incubation Time: 24, 48 h
Result: Reversed the effect of IGF1 on caspase-3 cleavage (Caspase-3 is activated in apoptotic cells and cleaves several cellular proteins, including PARP).

Western Blot Analysis[1]

Cell Line: MCF7 cells (IGF-1-induced)
Concentration: 33 nM
Incubation Time: 24 h
Result: Led to a decrease of phosphorylation for both β-chain and IRS-1.
体内研究
(In Vivo)

Dalotuzumab (h7C10; i.p.; 第一次 250 µg/小鼠，然后每周两次 125 µg/小鼠，持续 40 天) 显示对 MCF-7 和 A549 异种移植肿瘤模型的抗肿瘤作用[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Swiss Nude mice (MCF-7 and A549 xenograft tumor models)[1].
Dosage: 125 and 250 (first time) µg/mice
Administration: Intraperitoneal injection; 250 µg/mice for the first time, then 125 µg/mice twice weekly for 40 days
Result: Led to average tumor volume at 6 weeks post-cell injection was reduced by 70% and 72% in the MCF-7 and A549 models, respectively.
Clinical Trial
分子量

146.4 (kDa)

CAS 号
性状

液体

颜色

Colorless to light yellow

运输条件

Shipping with dry ice.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Dalotuzumab 相关分类

  • 摩尔计算器

  • 稀释计算器

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dalotuzumab1005389-60-5MK-0646 h7C10MK0646MK 0646IGF-1RApoptosisInsulin-like growth factor-1 receptorMCF7 cellNSCLC A459cancerproliferationECC-1 cellUSPC-1 cellapoptosisInhibitorinhibitorinhibit

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