1. Academic Validation
  2. COX-1 and COX-2 inhibition in horse blood by phenylbutazone, flunixin, carprofen and meloxicam: an in vitro analysis

COX-1 and COX-2 inhibition in horse blood by phenylbutazone, flunixin, carprofen and meloxicam: an in vitro analysis

  • Pharmacol Res. 2005 Oct;52(4):302-6. doi: 10.1016/j.phrs.2005.04.004.
C Beretta 1 G Garavaglia M Cavalli
Affiliations

Affiliation

  • 1 Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Via Celoria 10, 20133 Milan, Italy. carlo.beretta@unimi.it
Abstract

We report on the inhibitory activity of the NSAIDs meloxicam, carprofen, phenylbutazone and flunixin, on blood cyclooxygenases in the horse using in vitro enzyme-linked assays. As expected, comparison of IC50 indicated that meloxicam and carprofen are more selective inhibitors of COX-2 than phenylbutazone and flunixin; meloxicam was the most advantageous for horses of four NSAIDs examined. However at IC80, phenylbutazone (+134.4%) and flunixin (+29.7%) had greater COX-2 selectivity than at IC50, and meloxicam (-41.2%) and carprofen (-12.9%) had lower COX-2 selectivity than at IC50. We therefore propose that the selectivity of NSAIDs should be assessed at the 80% as well as 50% inhibition level.

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