1. Academic Validation
  2. Effect of recombinant human endostatin on the expression of c-Myc and bFGF in mouse gastric cancer cells

Effect of recombinant human endostatin on the expression of c-Myc and bFGF in mouse gastric cancer cells

  • Genet Mol Res. 2015 May 18;14(2):5258-65. doi: 10.4238/2015.May.18.17.
Z Y Guo 1 G D Yao 2 L P Fu 1 Z G Fu 1 B Hou 1
Affiliations

Affiliations

  • 1 Department of Radiotherapy, Handan Central Hospital, Affiliated Hospital of Hebei Medical University, Handan, Hebei, China.
  • 2 Department of Radiotherapy, Handan Central Hospital, Affiliated Hospital of Hebei Medical University, Handan, Hebei, China yaogendong@sina.com.
Abstract

The aim of this study was to observe the effects of re-combinant human endostatin on the proliferation and Apoptosis of mouse gastric Cancer cells, and explore some possible mechanisms of recom-binant human endostatin inhibition of Cancer. A murine gastric Cancer xenograft model was established. A total of 20 mice were divided into two groups (control and experimental groups). The expression of c-Myc and basic Fibroblast Growth Factor (bFGF) was determined by reverse transcription-polymerase chain reaction, Western blotting, and immu-nohistochemical staining methods. Tumor volume was measured and a growth curve was calculated. The tumor diameter in the experimental group was significantly smaller than that in the control group after treat-ment with endostatin for 21 days. The expression levels of c-Myc and bFGF in the experimental group were significantly lower than those of the control group (P < 0.05). There was a positive correlation between the expression of c-Myc and bFGF in the experimental group. Microvessel density was significantly inhibited in the experimental group (P < 0.05). These results demonstrated that recombinant human endostatin could in-hibit tumor metastasis by inhibition of the expression of c-Myc and bFGF in gastric Cancer tissue as well as by inhibition of angiogenesis.

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