1. Academic Validation
  2. In vitro activity of ceftobiprole on 440 Staphylococcus aureus strains isolated from bronchopulmonary infections

In vitro activity of ceftobiprole on 440 Staphylococcus aureus strains isolated from bronchopulmonary infections

  • Med Mal Infect. 2017 Mar;47(2):152-157. doi: 10.1016/j.medmal.2016.10.004.
E Hodille 1 L Delouere 2 C Bouveyron 2 H Meugnier 2 M Bes 3 A Tristan 3 F Laurent 3 F Vandenesch 3 G Lina 3 O Dumitrescu 3
Affiliations

Affiliations

  • 1 Centre de référence des staphylocoques, institut des agents infectieux, hospices civils de Lyon, 69495 Lyon, France; International Center for Infectiology Research, 69007 Lyon, France; CNRS UMR5308, Inserm U1111, École normale supérieure de Lyon, université Lyon 1, 69495 Lyon, France. Electronic address: elisabeth.hodille@chu-lyon.fr.
  • 2 Centre de référence des staphylocoques, institut des agents infectieux, hospices civils de Lyon, 69495 Lyon, France.
  • 3 Centre de référence des staphylocoques, institut des agents infectieux, hospices civils de Lyon, 69495 Lyon, France; International Center for Infectiology Research, 69007 Lyon, France; CNRS UMR5308, Inserm U1111, École normale supérieure de Lyon, université Lyon 1, 69495 Lyon, France.
Abstract

Objective: We assessed the in vitro activity of ceftobiprole on 440 Staphylococcus aureus clinical strains isolated from bronchopulmonary infections (2010-2014).

Methods: S. aureus isolates were characterized for methicillin resistance, PVL status, and clonal complex. All isolates were tested for minimal inhibitory concentrations (MIC) determination by broth microdilution method for ceftobiprole, ceftaroline fosamil, and comparator Antibiotics (linezolid, tigecycline, vancomycin, and daptomycin).

Results: A total of 325 (74%) strains were methicillin-susceptible S. aureus (MSSA) and 115 (26%) were methicillin-resistant S. aureus (MRSA); 105 (24%) S. aureus strains were PVL-positive, including 35.2% (37/105) MRSA and 64.8% (68/105) MSSA. Ceftobiprole was highly active against S. aureus with MIC90 of 1 mg/L, MICs ranging between 0.12 and 4mg/L (only one resistant strain, MIC of 4 mg/L). MIC50 and MIC90 were twice lower in MSSA than MRSA. Moreover, PVL+ MRSA were slightly more susceptible to ceftobiprole (MIC50 of 0.5 mg/L and MIC90 of 1 mg/L) than PVL- MRSA (MIC50 and MIC90 of 1 mg/L). The ceftobiprole-resistant strain was also resistant to ceftaroline fosamil and presented the D239L mutation in PBP2A. The comparator Antibiotics were equally active on the strains tested, with MIC90 of 0.5 mg/L for ceftaroline fosamil, tigecycline, and daptomycin; 1 mg/L for vancomycin; and 2 mg/L for linezolid.

Conclusions: Our results suggest that ceftobiprole is highly active against S. aureus and is an effective alternative to vancomycin or linezolid in the management of staphylococcal pneumonia. However, close monitoring of isolates should be maintained to prevent resistant strain diffusion.

Keywords

Bronchopulmonary infections; Ceftaroline fosamil; Ceftobiprole; Céftaroline; Céftobiprole; Infections broncho-pulmonaires; Staphylococcus aureus.

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