1. Academic Validation
  2. Adipose-derived stem cell-conditioned medium protects fibroblasts at different senescent degrees from UVB irradiation damages

Adipose-derived stem cell-conditioned medium protects fibroblasts at different senescent degrees from UVB irradiation damages

  • Mol Cell Biochem. 2020 Jan;463(1-2):67-78. doi: 10.1007/s11010-019-03630-8.
Shu Guo 1 Ting Wang 2 Shuangyi Zhang 3 Peng Chen 4 Zheng Cao 5 Wenqin Lian 6 Jiayan Guo 2 Yue Kang 7
Affiliations

Affiliations

  • 1 Department of Plastic Surgery, The First Hospital of China Medical University, 155 North Nanjing Street, Shenyang, 110001, Liaoning, People's Republic of China. sguo@cmu.edu.cn.
  • 2 Department of Plastic Surgery, The First Hospital of China Medical University, 155 North Nanjing Street, Shenyang, 110001, Liaoning, People's Republic of China.
  • 3 Department of Burn and Plastic Surgery, Shenyang Jishuitan Hospital, Shenyang, 110001, Liaoning, People's Republic of China.
  • 4 Department of Plastic and Aesthetic Surgery, Nanfang Hospital of Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China.
  • 5 Department of Burn and Plastic Surgery, 202 Hospital of the PLA, Shenyang, 110001, Liaoning, People's Republic of China.
  • 6 Department of Burn and Plastic Surgery, Xiangan Hospital of Xiamen University, Xiamen, 361100, Fujian, People's Republic of China.
  • 7 Educational Department, Liaoning Cancer Hospital, Shenyang, 110001, Liaoning, People's Republic of China.
Abstract

Adipose-derived stem cells (ADSCs) and their derivatives have aroused intense interest in fields of dermatological and aesthetic medicine. As a major component detected in ADSCs secretome, platelet-derived growth factor AA (PDGF-AA) has been reported mediating extracellular matrix deposition and remodeling, thus might contribute to its Anti-aging effect. On the basis of establishing an experimental model that simulate actual skin aging by exposing HDFs to both intrinsic and extrinsic aging factors, we pretreated human dermal fibroblasts (HDFs) with ADSC-conditioned medium (ADSC-CM) before being irradiated, aiming at exploring preventive effects of ADSCs secretome against aging damages. 48 h after irradiation, we detected cellular proliferation; β-galactosidase stain; mRNA expressions of MMP-1, MMP-9, and TIMP-1; and protein expressions of collagen I, collagen III, and elastin. Moreover, we detected related protein expression of PI3K/Akt signal pathway, which can be activated by PDGF-AA and was newly found to promote extracellular matrix protein synthesis. Concentration of PDGF-AA in the prepared ADSC-CM decreased over time and maintained excellent bioactivity at low temperature until the 11th week. ADSC-CM pretreatment can slightly or significantly improve cellular proliferative activity and reduce cellular senescence in irradiated HDFs. Besides, ADSC-CM pretreatment increased collagen I, collagen III, elastin, and TIMP-1 expressions but decreased MMP-1 and MMP-9 expressions both in irradiated and nonirradiated HDFs. ADSC-CM pretreatment significantly increased pAkt protein expression, and ECM protein expression greatly decreased in case of LY294002 application. The results were similar in three generations of HDFs, yet varied with different degrees. Generally, ADSC-CM we prepared demonstrates a certain degree of positive role in preventing HDFs from intrinsic and extrinsic aging damages and that PDGF-AA may contribute to making it become effective with some other components in ADSC-CM.

Keywords

Adipose-derived stem cell-conditioned medium; Anti-aging; Fibroblasts; Platelet-derived growth factor-AA; Replicative senescence; UVB irradiation.

Figures
Products