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  2. Simultaneous detection of 24 oral antidiabetic drugs and their metabolites in urine by liquid chromatography-tandem mass spectrometry

Simultaneous detection of 24 oral antidiabetic drugs and their metabolites in urine by liquid chromatography-tandem mass spectrometry

  • J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Mar 15;1141:122020. doi: 10.1016/j.jchromb.2020.122020.
Ying Hoo Lam 1 Mei Tik Leung 2 Chor Kwan Ching 1 Tony Wing Lai Mak 3
Affiliations

Affiliations

  • 1 Hospital Authority Toxicology Reference Laboratory, Princess Margaret Hospital, Hong Kong.
  • 2 Department of Pathology, Queen Elizabeth Hospital, Hong Kong.
  • 3 Hospital Authority Toxicology Reference Laboratory, Princess Margaret Hospital, Hong Kong. Electronic address: makwl@ha.org.hk.
Abstract

Drugs are the most frequent cause of hypoglycemia. Though the drug history is usually obvious in diabetic patients, the diagnosis could be a challenge in patients without a history of such exposure. Screening for oral antidiabetic drugs has been recommended as part of the hypoglycemia workup in patients without diabetes. Many published analytical methods of oral antidiabetic agents were usually of limited coverage and restricted to parent drugs only. In the current study, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) analytical system for the simultaneous detection of 24 oral antidiabetic drugs and their metabolites in urine was established and validated. The method covered both conventional as well as the newer antidiabetic drugs such as dipeptidyl peptidase-4 inhibitors and sodium-glucose cotransporter-2 inhibitors. Following sample preparation by solid phase extraction, analytes were detected by LC-MS/MS with multiple reaction monitoring triggered enhanced product ion scan. The method was successfully applied to 233 cases of unexplained hypoglycemia, with 83 oral antidiabetic drugs detected in 51 of the urine samples.

Keywords

Drug screening; Drug-induced hypoglycemia; LC-MS/MS; Oral antidiabetic drug; Urine analysis.

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