1. Academic Validation
  2. Targeted GSH-exhausting and hydroxyl radical self-producing manganese-silica nanomissiles for MRI guided ferroptotic cancer therapy

Targeted GSH-exhausting and hydroxyl radical self-producing manganese-silica nanomissiles for MRI guided ferroptotic cancer therapy

  • Nanoscale. 2020 Aug 20;12(32):16738-16754. doi: 10.1039/d0nr02396e.
Weidong Fei 1 Danfei Chen 2 Hongxia Tang 3 Chaoqun Li 3 Weizeng Zheng 4 Fengying Chen 1 Qianqian Song 1 Yunchun Zhao 1 Yu Zou 4 Caihong Zheng 1
Affiliations

Affiliations

  • 1 Department of Pharmacy, Women's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China. chzheng@zju.edu.cn.
  • 2 Department of Pediatrics, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, China.
  • 3 College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 311400, China.
  • 4 Department of Radiology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China. zouyuzju@zju.edu.cn.
Abstract

Ferroptosis, a cell death path induced by the generation of Reactive Oxygen Species (ROS), will cause the accumulation of lipid peroxides (PL-PUFA-OOH) and achieve potent tumor-regression. However, glutathione (GSH)-dependent Glutathione Peroxidase 4 (GPx4) can reduce PL-PUFA-OOH and antagonize the Ferroptosis inducing effect of ROS. Herein, folate-PEG modified dihydroartemisinin (DHA) loaded manganese doped mesoporous silica nanoparticles (described as nanomissiles) were constructed for integrating the effect of GSH exhaustion and ROS generation. After endocytosis by tumor cells, intracellular GSH triggered the degradation of nanomissiles, which allowed the simultaneous release of DHA and Fenton catalytic Mn2+ due to the redox reaction between the manganese-oxygen bonds and GSH. The degradation would lead to GSH exhaustion, activation of Mn2+-based magnetic resonance imaging (MRI), and DHA-driven ˙OH generation. The GSH-free environment inhibited the activity of GPx4 and enhanced the accumulation of PL-PUFA-OOH oxidized by ˙OH. Furthermore, the cooperative effects suppressed tumor metastasis by destroying the structure of polyunsaturated fatty acids in the cell membranes and showed potent antitumor activity. This innovative ferroptotic therapy integrating the GSH exhaustion and ROS generation will be a promising strategy for Cancer therapy.

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