2. Academic Validation
  3. Influences of galactose ligand on the uptake of TADF liposomes by HepG2 cells

Influences of galactose ligand on the uptake of TADF liposomes by HepG2 cells

  • Photodiagnosis Photodyn Ther. 2020 Dec;32:102014. doi: 10.1016/j.pdpdt.2020.102014.
Chunyue Wang 1,  Zhong Chen 2,  Xuefeng Tang 2,  Xiaoying Liu 2,  Wang Na 2,  Wenhua Li 3,  Ting Liu 4
Affiliations

Affiliations

  • 1 Fuwai Hospital Chinese Academy of Medical Sciences &Peking Union Medical College, National Center for Cardiovascular Diseases, State Key Laboratory of Cardiovascular Disease, China; Daqing Campus of Harbin Medical University, 1 Xinyang Rd Daqing, 163319, China.
  • 2 Daqing Campus of Harbin Medical University, 1 Xinyang Rd Daqing, 163319, China.
  • 3 School of Pharmacy, Harbin University of Commerce, 138, Tong Da Street, Harbin 150076, China; Daqing Campus of Harbin Medical University, 1 Xinyang Rd Daqing, 163319, China. Electronic address: 461892270@qq.com.
  • 4 Daqing Campus of Harbin Medical University, 1 Xinyang Rd Daqing, 163319, China. Electronic address: 398300987@qq.com.
PMID: 32950730 DOI: 10.1016/j.pdpdt.2020.102014
Abstract

Glucose is the main energy substance to drive the physiological events of the cell.. Malignant cells exhibit a much higher rate of glycolysis than healthy cells to relieve the increased needs of energy. The higher metabolic rate induces the over-expression of the Glucose Transporter (GLUT) to transport more glucose into malignant cells. Our research regarded overexpressive GLUT as a target of nanoparticles. Substrate of GLUT galactose conjugated Polyethylene glycol-Distearyl phosphatidyl ethanolamine (PEG-DSPE) as a kind of ligand was selected to modified Liposome. Thermally activated delayed fluorescence (TADF) was encapsulated as fluorescent probe to evaluate its abilities of targeting malignant cells, and the results of confocal microscopy and flow cytometry demonstrated that Galactose-PEG-DSPE modified Liposome had the stronger efficiency of cellular uptake by HepG2 cells compared with Blank-PEG-DSPE modified Liposome. The effect of GLUT1 Inhibitor on cellular uptake of Galactose-PEG-DSPE modified liposomes showed that the mechanism might be relative to Warburg effect causing GLUT overexpression.

Keywords

GLUT; Galactose; HepG2 cells; Liposome; Warburg effect.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-19331
    99.97%, Glut1抑制剂
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