2. Academic Validation
  3. Classical swine fever virus infection suppresses claudin-1 expression to facilitate its replication in PK-15 cells

Classical swine fever virus infection suppresses claudin-1 expression to facilitate its replication in PK-15 cells

  • Microb Pathog. 2021 Aug;157:105012. doi: 10.1016/j.micpath.2021.105012.
Xiangmin Wang 1,  Yu'ai Yang 1,  Xiaoying Yang 1,  Xiao Liu 2,  Xiaochun Wang 1,  Libo Gao 1,  Chao Yang 1,  Rui Lan 1,  Junlong Bi 3,  Qian Zhao 4,  Guishu Yang 1,  Jing Wang 5,  Yingbo Lin 6,  Jianping Liu 7,  Gefen Yin 8
Affiliations

Affiliations

  • 1 College of Animal Veterinary Medicine, Yunnan Agricultural University, Kunming, 650201, Yunnan, China.
  • 2 College of Animal Veterinary Medicine, Yunnan Agricultural University, Kunming, 650201, Yunnan, China; Department of Oncology-Pathology, Karolinska Institutet, 17176, Stockholm, Sweden.
  • 3 Institute of Science and Technology, Chuxiong Normal University, 546 Lucheng South Rd, Chuxiong, 675000, Yunnna, China.
  • 4 Center for Animal Disease Control and Prevention, Chuxiong, 675000, Yunnan, China.
  • 5 School of Clinical Medicine, Dali University, Dali, 671003, Yunnan, China.
  • 6 Department of Oncology-Pathology, Karolinska Institutet, 17176, Stockholm, Sweden.
  • 7 School of Clinical Medicine, Dali University, Dali, 671003, Yunnan, China. Electronic address: jianping.liu@ki.se.
  • 8 College of Animal Veterinary Medicine, Yunnan Agricultural University, Kunming, 650201, Yunnan, China. Electronic address: yingefen0616@163.com.
PMID: 34062228 DOI: 10.1016/j.micpath.2021.105012
Abstract

Classical swine fever (CSF) is one of the most epidemic viral diseases in swine industry. The causative pathogen is CSF virus (CSFV), a small enveloped RNA virus of Flaviviridae family. Claudin-1 was reported to be involved in the infections of a number of viruses, including many from Flaviviridae family, but no studies have investigated the role of porcine claudin-1 during CSFV Infection in PK-15 cells. In this study, on the one hand, we demonstrated that CSFV Infection reduced the claudin-1 expression at both mRNA and protein levels; on the other hand, CSFV Infection was enhanced after claudin-1 knockdown, but inhibited by claudin-1 overexpression in a dose-dependent manner. Furthermore, negative correlation was demonstrated between the claudin-1 expression and CSFV titer. In conclusion, claudin-1 might be a barrier for CSFV Infection in PK-15 cells, while CSFV bypasses the barrier through lysosome mediated degradation of claudin-1, which could be repressed by bafilomycin A1. Although the elaborate mechanisms how claudin-1 plays its roles in CSFV Infection require further investigations, this study may advance our understanding of the molecular host-pathogen interaction mechanisms underlying CSFV Infection and suggests enhancement of porcine claudin-1 as a potential preventive or therapeutic strategy for CSF control.

Keywords

Classic swine fever virus; Claudin-1; Infection; Lysosome; NF-κB; PK-15 cells.

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