1. Academic Validation
  2. Promotion of gastric tumor initiating cells in a 3D collagen gel culture model via YBX1/SPP1/NF-κB signaling

Promotion of gastric tumor initiating cells in a 3D collagen gel culture model via YBX1/SPP1/NF-κB signaling

  • Cancer Cell Int. 2021 Nov 10;21(1):599. doi: 10.1186/s12935-021-02307-x.
Shuangya Deng 1 Lun Li 1 Shu Xu 1 Xiaobo Wang 1 Tong Han 2
Affiliations

Affiliations

  • 1 Department of General Surgery, The Second Xiangya Hospital of Central South University, Changsha, China.
  • 2 Department of General Surgery, The Second Xiangya Hospital of Central South University, Changsha, China. hantong@csu.edu.cn.
Abstract

Background: The high potential for tumor recurrence and chemoresistance is a major challenge of clinical gastric Cancer treatment. Increasing evidence suggests that the presence of tumor initiating cells (TICs) is the principal cause of tumor recurrence and chemoresistance. However, the underlying mechanism of TIC development remains controversial.

Methods: To identify novel molecular pathways in gastric Cancer, we screened the genomic expression profile of 155 gastric Cancer patients from the TCGA database. We then described an improved 3D collagen I gels and tested the effects of collagen on the TIC phenotype of gastric cells using colony formation assay, transwell assay, and nude mouse models. Additionally, cell Apoptosis assay was performed to examine the cytotoxicity of 5-fluorine and paclitaxel on gastric Cancer cells cultured in 3D collagen I gels.

Results: Elevated expression of type I collagen was observed in tumor tissues from high stage patients (stage T3-T4) when compared to the low stage group (n=10, stage T1-T2). Furthermore, tumor cells seeded in a low concentration of collagen gels acquired TIC-like phenotypes and revealed enhanced resistance to chemotherapeutic agents, which was dependent on an Integrin β1 (ITGB1)/Y-box Binding Protein 1 (YBX1)/Secreted Phosphoprotein 1 (SPP1)/NF-κB signaling pathway. Importantly, inhibition of ITGB1/NF-κB signaling efficiently reversed the chemoresistance induced by collagen and promoted Anticancer effects in vivo.

Conclusions: Our findings demonstrated that type I collagen promoted TIC-like phenotypes and chemoresistance through ITGB1/YBX1/SPP1/NF-κB pathway, which may provide novel insights into gastric Cancer therapy.

Keywords

Collagen; Gastric cancer; Integrin β1 signaling; Secreted phosphoprotein 1 (SPP1); Tumor initiating cells (TICs); Y-box binding protein 1 (YBX1).

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