1. Academic Validation
  2. Bisphenol F suppresses insulin-stimulated glucose metabolism in adipocytes by inhibiting IRS-1/PI3K/AKT pathway

Bisphenol F suppresses insulin-stimulated glucose metabolism in adipocytes by inhibiting IRS-1/PI3K/AKT pathway

  • Ecotoxicol Environ Saf. 2022 Feb;231:113201. doi: 10.1016/j.ecoenv.2022.113201.
Huiling Chen 1 Jiangbin Li 2 Yanchao Zhang 1 Wei Zhang 1 Xing Li 1 Huanwen Tang 1 Yungang Liu 3 Tianlan Li 1 Haoqi He 1 Bohai Du 1 Li Li 4 Ming Shi 5
Affiliations

Affiliations

  • 1 Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan 523808, Guangdong Province, China.
  • 2 School of Medical Technology, Guangdong Medical University, Dongguan 523808, Guangdong Province, China.
  • 3 Department of Toxicology, School of Public Health, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
  • 4 Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan 523808, Guangdong Province, China. Electronic address: lily2017@gdmu.edu.cn.
  • 5 Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan 523808, Guangdong Province, China; Dongguan Liaobu Hospital, Dongguan 523808, Guangdong Province, China. Electronic address: shiming@gdmu.edu.cn.
Abstract

Obesity is one of the risk factors of metabolic diseases. Decreased sensitivity to Insulin or impairment of the Insulin signaling pathway may affect the metabolism of adipose tissue. Bisphenol F (BPF) has been widely used in various products as a substitute for bisphenol A (BPA). BPA has been defined as "obesogen". However, knowledge about the correlation between BPF and obesity is very limited. This study was aimed to explore the effects of BPF on glucose metabolism and Insulin sensitivity in mammalian tissues, using a mouse 3T3-L1 adipocyte line as the model. Differentiated 3T3-L1 adipocytes were treated with BPF at various concentrations for 24 h or 48 h, followed by the measurement of cell viability, lipid accumulation, expression levels of adipocytokines, glucose consumption, and impairment of the Insulin signaling pathway. The results indicated that BPF had no effect on the size of 3T3-L1 adipocytes, but the expression of Leptin, Adiponectin and apelin was decreased, while that of chemerin and resistin was increased after 48 h of BPF treatment. Moreover, BPF inhibited the glucose consumption, the expression of GLUT4, and its translocation to the plasma membranes in 3T3-L1 adipocytes. Western blot analysis indicated that the activation of IRS-1/PI3K/Akt signaling pathway was inhibited by BPF, which resulted in reduced GLUT4 translocation. In conclusion, our data suggest that exposure of adipocytes to BPF may alter the expression of calorie metabolism-related adipokines and suppress insulin-stimulated glucose metabolism by impairing the Insulin signaling (IRS-1/PI3K/Akt) pathway.

Keywords

Adipocyte; Adipokine; Bisphenol F; Glucose metabolism; IRS-1/PI3K/Akt pathway.

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