2. Academic Validation
  3. Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 Regulation

Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 Regulation

  • Front Genet. 2022 Mar 3;12:831420. doi: 10.3389/fgene.2021.831420.
Yongchang Yang 1 2,  Li Miao 3,  Shuai Chang 2,  Qiuli Zhang 4,  Lijuan Yu 1 5,  Ping He 6,  Yue Zhang 1 7,  Weixiao Fan 1 5,  Jie Liu 2,  Xiaoke Hao 1 5 8
Affiliations

Affiliations

  • 1 Institute of Laboratory Medicine Center of Chinese People's Liberation Army (PLA), Xijing Hospital, Fourth Military Medical University (Air Force Medical University), Xi'an, China.
  • 2 Department of Clinical Laboratory, Seventh Medical Center of Chinese PLA General Hospital, Beijing, China.
  • 3 Department of Stomatology, Seventh Medical Center of Chinese PLA General Hospital, Beijing, China.
  • 4 Department of Blood Transfusion, Seventh Medical Center of Chinese PLA General Hospital, Beijing, China.
  • 5 Department of Clinical Laboratory Medicine, Xijing Hospital, Fourth Military Medical University (Air Force Medical University), Xi'an, China.
  • 6 BMD Testing Room, Department of Orthopedic, Seventh Medical Center of Chinese PLA General Hospital, Beijing, China.
  • 7 Department of Clinical Laboratory, Air Force Hospital in the Northern Theater Command, Shenyang, China.
  • 8 College of Medicine, Northwest University, Xi'an, China.
PMID: 35308164 DOI: 10.3389/fgene.2021.831420
Abstract

Postmenopausal osteoporosis (PMOP) is a systemic metabolic bone disease in postmenopausal women. It has been known that long non-coding RNAs (lncRNAs) play a regulatory role in the progression of osteoporosis. However, the mechanism underlying the effects of exosome-derived lncRNA on regulating the occurrence and development of PMOP remains unclear. Exosomes in the serum of patients PMOP were collected and identified. RNA sequencing was performed to obtain the expression profile of exosome-derived lncRNAs in the serum of PMOP patients. RNA sequencing identified 26 differentially expressed lncRNAs from the exosomes between healthy people and PMOP patients. Among them, the expression of TCONS_00072128 was dramatically down-regulated. A co-location method was employed and searched its potential target gene Caspase 8. TCONS_00072128 knockdown notably decreased the expression of Caspase 8, while the osteogenic differentiation of BMSCs was also reduced. Reversely, TCONS_00072128 overexpression enhanced Caspase 8 expression and osteogenic differentiation of BMSCs. Moreover, the continuous expression of Caspase 8 regulated by TCONS_00072128 significantly activated inflammation pathways including NLRP3 signaling and NF-κB signaling. Simultaneously, RIPK1 which has emerged as a promising therapeutic target for the treatment of a wide range of human neurodegenerative, autoimmune, and inflammatory diseases, was also phosphorylated. The results of the present study suggested that exosome-derived lncRNA TCONS_00072128 could promote the progression of PMOP by regulating Caspase 8. In addition, Caspase 8 expression in BMSCs was possible to be a key regulator that balanced cell differentiation and inflammation activation.

Keywords

PMOP; caspase 8; exosome; lncRNA; osteogenic differentiation.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-101297
    ≥98.0%, Caspase-8抑制剂
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