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  2. The canonical Wnt/β-catenin signaling pathway facilitates pseudorabies virus proliferation and enhances virus-induced autophagy

The canonical Wnt/β-catenin signaling pathway facilitates pseudorabies virus proliferation and enhances virus-induced autophagy

  • Vet Microbiol. 2022 Sep;272:109502. doi: 10.1016/j.vetmic.2022.109502.
Chongyang Wang 1 Ruochen Hu 1 Liuyuan Duan 1 Qili Hou 1 Mengqing Yang 1 Ting Wang 1 Haijin Liu 1 Sa Xiao 1 Ruyi Dang 1 Juan Wang 1 Xinglong Wang 1 Shuxia Zhang 2 Zengqi Yang 3
Affiliations

Affiliations

  • 1 College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
  • 2 College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China. Electronic address: zhangshuxia316@163.com.
  • 3 College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China. Electronic address: yzq8162@163.com.
Abstract

Pseudorabies virus (PRV) is a swine herpesvirus with a broad host range that causes significant economic losses worldwide. The Wnt/β-catenin signaling pathway is reportedly involved in multiple viruses' proliferation. In this study, we demonstrated that PRV Infection significantly activated the Wnt/β-catenin signaling and promoted the nuclear translocation of β-catenin. Applying specific chemical inhibitors (FH535 and iCRT14) caused a remarkable decrease in PRV titers in various cell lines. Knockdown of β-catenin by siRNA also reduced the proliferation of PRV. On the contrary, treatment with lithium chloride (LiCl), an inhibitor of GSK3β, stimulated the Wnt/β-catenin signaling pathway and enhanced the PRV proliferation. Similarly, overexpression of β-catenin promoted PRV proliferation and reversed the Antiviral effect of FH535. Moreover, LiCl promoted PRV-induced Autophagy, whereas FH535 and iCRT14 showed converse effects. These findings suggest that PRV Infection stimulates the canonical Wnt/β-catenin signaling pathway, facilitating PRV proliferation and regulating virus-induced Autophagy. These data also provide potential targets for developing Antiviral agents against PRV.

Keywords

Autophagy; PRV; Wnt/β-catenin signaling pathway.

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