2. Academic Validation
  3. Qinling liquid ameliorates renal immune inflammatory damage via activating autophagy through AMPK/Stat3 pathway in uric acid nephropathy

Qinling liquid ameliorates renal immune inflammatory damage via activating autophagy through AMPK/Stat3 pathway in uric acid nephropathy

  • Cytokine. 2023 Jan 9;163:156120. doi: 10.1016/j.cyto.2022.156120.
Jie Wang 1,  Xiangwei Bu 2,  Xinping Qiu 3,  Xiuyuan Zhang 1,  Jianhua Gui 1,  Honghong Zhang 4,  Yun Wang 5,  Chen Wang 3,  Fengxian Meng 6
Affiliations

Affiliations

  • 1 Department of Endocrinology, Shunyi Hospital, Beijing Traditional Chinese Medicine Hospital, Beijing 101300, China.
  • 2 Department of Endocrinology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
  • 3 Scientific Research Department, Shunyi Hospital, Beijing Traditional Chinese Medicine Hospital, Beijing 101300, China.
  • 4 Department of Rheumatology, Shunyi Hospital, Beijing Traditional Chinese Medicine Hospital, Beijing 101300, China.
  • 5 Department of Cardiology, Shunyi Hospital, Beijing Traditional Chinese Medicine Hospital, Beijing 101300, China.
  • 6 Department of Rheumatology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China. Electronic address: mfx0823@126.com.
PMID: 36630870 DOI: 10.1016/j.cyto.2022.156120
Abstract

Background: Excessive deposition of uric acid (UA) is one of the risk factors for kidney damage. Qinling liquid (QL) has a certain therapeutic effect on uric acid nephropathy (UAN), but its regulation mechanism is still unclear.

Methods: UAN rat models and UA induced rat renal tubular epithelial cells (NRK-52E) were constructed to evaluate the functional roles of QL. We firstly evaluated the kidney function and the degree of kidney damage in rats after QL treatment. Then, effects of QL on Autophagy and NLRP3 inflammasome activation were assessed. Moreover, the regulation of QL in AMPK and STAT3 phosphorylation levels and the relationship among Autophagy, AMPK/STAT3 pathway and NLRP3 inflammasomes were determined.

Results: QL could alleviate the inflammatory damage in UAN rats and promote the activation of Autophagy. In addition, QL suppressed UA-induced activation of NLRP3 inflammasomes in rat renal tubular epithelial cells, which was partially reversed by Autophagy Inhibitor. Further, AMPK/STAT3 axis-mediated Autophagy participated in the regulation of UA-induced NLRP3 inflammasome activation in NRK-52E cells. Finally, we confirmed that inhibiting AMPK/STAT3 pathway partly deteriorated the ameliorating effect of QL on renal immune inflammatory injury in UAN rats.

Conclusion: Through in vivo and in vitro experiments, we found that QL promotes Autophagy by activating the AMPK/STAT3 pathway, thereby improving renal immune inflammatory injury in UAN.

Keywords

AMPK/Stat3 pathway; Autophagy; Qinling liquid; Uric acid nephropathy.

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