1. Academic Validation
  2. The Inactivation of Hippo Signaling Pathway Promotes the Development of Adenomyosis by Regulating EMT, Proliferation, and Apoptosis of Cells

The Inactivation of Hippo Signaling Pathway Promotes the Development of Adenomyosis by Regulating EMT, Proliferation, and Apoptosis of Cells

  • Reprod Sci. 2023 Mar 20. doi: 10.1007/s43032-023-01189-w.
Tingting Jin 1 2 Mengqi Li 1 2 Ting Li 1 2 Simiao Yan 1 2 Qingzhen Ran 3 Wanqun Chen 4 5
Affiliations

Affiliations

  • 1 Department of Biochemistry and Molecular Biology, School of Medicine, Jinan University, Guangzhou, 510632, Guangdong, China.
  • 2 Engineering Technology Research Center of Drug Development for Small Nucleic Acids, Guangzhou, 510632, Guangdong, China.
  • 3 Department of Gynecology, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 510120, Guangdong, China. 1902518646@qq.com.
  • 4 Department of Biochemistry and Molecular Biology, School of Medicine, Jinan University, Guangzhou, 510632, Guangdong, China. tchwanq@jnu.edu.cn.
  • 5 Engineering Technology Research Center of Drug Development for Small Nucleic Acids, Guangzhou, 510632, Guangdong, China. tchwanq@jnu.edu.cn.
Abstract

Adenomyosis is a benign gynecological disease. The pathogenesis of adenomyosis is still unclear. The Hippo signaling pathway is highly conserved in vivo and associated with endometriosis and various cancers. Our objective was to study the expression of Hippo signaling pathway-related proteins in the uterus of mice with and without adenomyosis. We also sought to determine the relationship between the Hippo signaling pathway and cell migration, invasion, proliferation, and Apoptosis in adenomyosis. The inactivation of Hippo signaling pathway and abnormal expression of EMT-related proteins were observed in mice with adenomyosis. In vitro, the YAP Inhibitor verteporfin can inhibit the proliferation and migration of Ishikawa cells and promote Apoptosis, while inhibiting the EMT process. In addition, intraperitoneal injection of verteporfin inhibits EMT process and proliferation and promotes Apoptosis of cells in the uterus of adenomyosis mice. It suggests that the Hippo signaling pathway participates in the EMT, proliferation, and Apoptosis of cells in adenomyosis. In conclusion, these results suggest that Hippo signaling pathway may be involved in the development of adenomyosis by regulating EMT, proliferation, and Apoptosis of cells, which provide a potential target for the treatment of adenomyosis.

Keywords

Adenomyosis (ADM); Epithelial-mesenchymal transition (EMT); Hippo signaling pathway; YAP (Yes-associated protein).

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