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  3. Cordycepin protects islet β-cells against glucotoxicity and lipotoxicity via modulating related proteins of ROS/JNK signaling pathway

Cordycepin protects islet β-cells against glucotoxicity and lipotoxicity via modulating related proteins of ROS/JNK signaling pathway

  • Biomed Pharmacother. 2023 Apr 24;163:114776. doi: 10.1016/j.biopha.2023.114776.
Baiyi Yan 1,  Yanchun Gong 1,  Wei Meng 2,  Huizhen Sun 3,  Wenxi Li 1,  Kaizhi Ding 1,  Caixia Dang 1,  Xiaofei Gao 1,  Wei Sun 1,  Chunhua Yuan 1,  Songhua Wang 4,  Li-Hua Yao 5
Affiliations

Affiliations

  • 1 School of Life Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR China.
  • 2 Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR China.
  • 3 School of Life Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR China; Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR China; School of Sport Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR China.
  • 4 Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR China; School of Sport Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR China. Electronic address: wangsonghua18@126.com.
  • 5 School of Life Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR China; School of Sport Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi 330013, PR China. Electronic address: yaolh7905@163.com.
PMID: 37100012 DOI: 10.1016/j.biopha.2023.114776
Abstract

Type 2 diabetes mellitus (T2DM) is a common and multiple endocrine Metabolic Disease. When pancreatic β cell in case of dysfunction, the synthesis and secretion of Insulin are reduced. This study is to explore the effect of cordycepin (the molecular formula C10H13N5O3), a natural adenosine isolated from Cordyceps militaris, on high glucose/lipid-induced glucotoxicity and lipotoxicity in INS-1 cells. Our results showed that cordycepin improved cell viability, improved cell energy metabolism and promoted Insulin synthesis and secretion. The mechanism may be related to that cordycepin reduces intracellular Reactive Oxygen Species (ROS), increases ATP content in cells, causes membrane depolarization and balances the steady state of Ca2+ concentration, cordycepin inhibits cell Apoptosis, which may be related to the downregulation of proteins level of c-Jun N-terminal kinases (JNK) phosphorylation, cytochrome c (Cyt-c), Cleaved Capase-3, the mRNA level of JNK, Cyt-c, Capase-3 and upregulation of proteins/mRNA level of pancreatic and duodenal homeobox factor-1 (PDX-1). These results suggest that cordycepin can inhibit cell Apoptosis and protect cell number by downregulating ROS/JNK mitochondrial Apoptosis pathway under high glucose/lipid environment, thereby improving the function of pancreatic islet cells, providing a theoretical basis for the related research on the prevention and control of cordycepin on T2DM.

Keywords

Apoptosis; Cordycepin; Diabetes; Glucotoxicity; INS-1 cell; Lipotoxicity.

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