1. Academic Validation
  2. Geniposide suppressed OX-LDL-induced osteoblast apoptosis by regulating the NRF2/NF-κB signaling pathway

Geniposide suppressed OX-LDL-induced osteoblast apoptosis by regulating the NRF2/NF-κB signaling pathway

  • J Orthop Surg Res. 2023 Aug 30;18(1):641. doi: 10.1186/s13018-023-04125-5.
Yaosheng Xiao # 1 2 Shanshan Zhang # 3 Yongjun Ye 2 Jincai Chen 2 Youjia Xu 4
Affiliations

Affiliations

  • 1 Medical College of Soochow University, Suzhou, 215123, China.
  • 2 Department of Orthopedics, First Affiliated Hospital of Gannan Medical University, Ganzhou, 341000, China.
  • 3 College of Pharmacy, Gannan Medical University, Ganzhou, 341000, China.
  • 4 Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou, 215004, China. xuyoujia@suda.edu.cn.
  • # Contributed equally.
Abstract

Background: Osteoporosis (OP), due to microarchitectural alterations, is associated with decreased bone mass, declined strength, and increased fracture risk. Increased osteoblast Apoptosis contributes to the progression of OP. Natural compounds from herbs provide a rich resource for drug screening. Our previous investigation showed that geniposide (GEN), an effective compound from Eucommia ulmoides, could protect against the pathological development of OP induced by Cholesterol accumulation.

Methods: The rat OP models were duplicated. Dual-energy X-ray absorptiometry, hematoxylin and eosin staining, and immunohistochemistry were used to evaluate bone changes. TUNEL/DAPI staining assays were used for cell Apoptosis detection. Protein expression was determined by western blotting assays.

Results: A high-fat diet promoted OP development in vivo, and OX-LDL stimulated osteoblast Apoptosis in vitro. GEN exhibited protective activities against OX-LDL-induced osteoblast Apoptosis by increasing the NRF2 pathway and decreasing the NF-κB pathway. PDTC, an NF-κB Inhibitor, could further promote the biological functions of GEN. In contrast, ML385, an NRF2 inhibitor, might eliminate GEN's protection.

Conclusion: GEN suppressed OX-LDL-induced osteoblast Apoptosis by regulating the NRF2/NF-κB signaling pathway.

Keywords

Apoptosis; Geniposide; NRF2/NF-κB; Osteoblast; Osteoporosis.

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