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  3. Chicken muscle antibody array reveals the regulations of LDHA on myoblast differentiation through energy metabolism

Chicken muscle antibody array reveals the regulations of LDHA on myoblast differentiation through energy metabolism

  • Int J Biol Macromol. 2023 Oct 25:127629. doi: 10.1016/j.ijbiomac.2023.127629.
Zihao Zhang 1,  Wen Luo 2,  Genghua Chen 3,  Jiahui Chen 3,  Shudai Lin 4,  Tuanhui Ren 3,  Zetong Lin 3,  Changbin Zhao 3,  Huaqiang Wen 3,  Qinghua Nie 5,  Xun Meng 6,  Xiquan Zhang 7
Affiliations

Affiliations

  • 1 Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affair, South China Agricultural University, Guangzhou 510642, China; State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou 510642, China; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
  • 2 Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affair, South China Agricultural University, Guangzhou 510642, China; State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou 510642, China; Lingnan Guangdong Laboratory of Agriculture, South China Agricultural University, Guangzhou 510642, China; Department of Orthaepedics and Traumatology, The Chinese University of Hong Kong, Hong Kong 999077, China.
  • 3 Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affair, South China Agricultural University, Guangzhou 510642, China; State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou 510642, China.
  • 4 College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
  • 5 Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affair, South China Agricultural University, Guangzhou 510642, China; State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou 510642, China; Lingnan Guangdong Laboratory of Agriculture, South China Agricultural University, Guangzhou 510642, China.
  • 6 School of Life Sciences, Northwest University, Xi'an 710069, China; Abmart, 333 Guiping Road, Shanghai 200033, China. Electronic address: xun.meng@ab-mart.com.
  • 7 Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affair, South China Agricultural University, Guangzhou 510642, China; State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou 510642, China; Lingnan Guangdong Laboratory of Agriculture, South China Agricultural University, Guangzhou 510642, China. Electronic address: xqzhang@scau.edu.cn.
PMID: 37890747 DOI: 10.1016/j.ijbiomac.2023.127629
Abstract

Myoblast proliferation and differentiation are highly dynamic and regulated processes in skeletal muscle development. Given that proteins serve as the executors for the majority of biological processes, exploring key regulatory factors and mechanisms at the protein level offers substantial opportunities for understanding the skeletal muscle development. In this study, a total of 607 differentially expressed proteins between proliferation and differentiation in myoblasts were screened out using our chicken muscle antibody array. Biological function analysis revealed the importance of energy production processes and compound metabolic processes in myogenesis. Our antibody array specifically identified an upregulation of LDHA during differentiation, which was associated with the energy metabolism. Subsequent investigation demonstrated that LDHA promoted the glycolysis and TCA cycle, thereby enhancing myoblasts differentiation. Mechanistically, LDHA promotes the glycolysis and TCA cycle but inhibits the ETC oxidative phosphorylation through enhancing the NADH cycle, providing the intermediate metabolites that improve the myoblasts differentiation. Additionally, increased glycolytic ATP by LDHA induces Akt phosphorylation and activate the PI3K-Akt pathway, which might also contribute to the promotion of myoblasts differentiation. Our studies not only present a powerful tool for exploring myogenic regulatory factors in chicken muscle, but also identify a novel role for LDHA in modulating myoblast differentiation through its regulation of cellular NAD+ levels and subsequent downstream effects on mitochondrial function.

Keywords

Energy metabolism regulation; Muscle antibody array; Myoblast differentiation.

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