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  2. Biotin-conjugated Ru(II) complexes with AIE characteristics as mitochondria-targeted photosensitizers for enhancing photodynamic therapy by disrupting cellular redox balance

Biotin-conjugated Ru(II) complexes with AIE characteristics as mitochondria-targeted photosensitizers for enhancing photodynamic therapy by disrupting cellular redox balance

  • Eur J Med Chem. 2023 Nov 23:264:115985. doi: 10.1016/j.ejmech.2023.115985.
Lai Wei 1 Xiangdong He 1 Deming Zhao 1 Martha Kandawa-Shultz 2 Guoqiang Shao 3 Yihong Wang 4
Affiliations

Affiliations

  • 1 School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, China.
  • 2 Department of Chemistry and Biochemistry, University of Namibia, Windhoek, 13301, Namibia.
  • 3 Department of Nuclear Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing 211166, China. Electronic address: guoqiangshao@163.com.
  • 4 School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, China. Electronic address: yihongwang@seu.edu.cn.
Abstract

The potential use of Ru(II) complexes as photosensitizers (PSs) in photodynamic therapy (PDT) has gained significant attention. In comparison with fluorophores with aggregation-caused quenching (ACQ), fluorophores with aggregation-induced emission (AIE) characteristics exhibit sustained fluorescence and dispersibility in aqueous solutions. PSs with AIE characteristics have received much attention in recent years. Herein, we reported two novel biotin-conjugated Ru(II) polypyridyl complexes (Ru1 and Ru2) with AIE characteristics. When exposed to 460 nm (10 mW cm-2) LIGHT, Ru1 and Ru2 exhibited outstanding photostability and photocatalytic activity. Ru1 and Ru2 could efficiently generate singlet oxygen and induce pUC19 DNA photolysis when exposed to 460 nm LIGHT. Interestingly, both Ru1 and Ru2 also functioned as catalysts for NADH oxidation when exposed to 460 nm LIGHT. The presence of biotin fragments in Ru1 and Ru2 enhanced the specific uptake of these complexes by tumor cells. Both complexes showed minimal toxicity to selected cells in the dark. Nevertheless, the phototoxicity of both complexes significantly increased upon 460 nm LIGHT irradiation for 15 min. Further experiments revealed that Ru2 primarily accumulated in mitochondria and might bind to mitochondrial DNA. Under 460 nm LIGHT irradiation, Ru2 induced the generation of Reactive Oxygen Species (ROS) and NADH depletion disrupting intracellular redox homeostasis in A549 cells, activating the mitochondrial Apoptosis pathway resulting in up-regulation of apoptotic marker Caspase-3, effectively damaged A549 cell DNA and arrested A549 cell cycle in the S phase. In vivo anti-tumor experiments were conducted to assess the effects of Ru2 on tumor growth in A549 tumor-bearing mice. The results showed that Ru2 effectively inhibited tumor growth under 460 nm LIGHT irradiation conditions. These findings indicate that Ru2 has great potential as a targeted photosensitizer for mitochondrial targeting imaging and photodynamic therapy of tumors.

Keywords

Aggregation-induced emission (AIE); Biotin; Mitochondria targeting; Photodynamic therapy (PDT); Ruthenium(II) complexes.

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