2. Academic Validation
  3. Functional analysis of the ube3a response in Japanese flounder (Paralichthys olivaceus) to CSBV infection

Functional analysis of the ube3a response in Japanese flounder (Paralichthys olivaceus) to CSBV infection

  • Fish Shellfish Immunol. 2024 Mar 8:109495. doi: 10.1016/j.fsi.2024.109495.
Huaxing Su 1,  Yitong Zhang 2,  Zhongwei He 2,  Yucong Yang 2,  Yuqin Ren 2,  Wei Cao 2,  Yufeng Liu 2,  Jiangong Ren 2,  Yufen Wang 2,  Guixing Wang 2,  Chunguang Gong 3,  Jilun Hou 4
Affiliations

Affiliations

  • 1 Hebei Key Laboratory of the Bohai Sea Fish Germplasm Resources Conservation and Utilization, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao, 066100, China; Ocean College, Hebei Agricultural University, Qinhuangdao, 066009, China; Bohai Sea Fishery Research Center, Chinese Academy of Fishery Sciences, Qinhuangdao, 066100, China.
  • 2 Hebei Key Laboratory of the Bohai Sea Fish Germplasm Resources Conservation and Utilization, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao, 066100, China; Bohai Sea Fishery Research Center, Chinese Academy of Fishery Sciences, Qinhuangdao, 066100, China.
  • 3 Ocean College, Hebei Agricultural University, Qinhuangdao, 066009, China. Electronic address: gongcg2005@163.com.
  • 4 Hebei Key Laboratory of the Bohai Sea Fish Germplasm Resources Conservation and Utilization, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao, 066100, China; Bohai Sea Fishery Research Center, Chinese Academy of Fishery Sciences, Qinhuangdao, 066100, China. Electronic address: houjl@bces.ac.cn.
PMID: 38461876 DOI: 10.1016/j.fsi.2024.109495
Abstract

Ube3a is a member of the E3 ubiquitin ligase HECTc family, and its role has been established in neurodevelopmental disorders. However, studies on its role in Japanese flounder are scarce. Thus, in this study, the ube3a of Japanese flounder was cloned, and its role in conferring resistance against Chinook salmon bafnivirus (CSBV) was analyzed. Japanese flounder ube3a encoded a protein containing 834 Amino acids. Interestingly, its homology with the Atlantic halibut was determined to be 94%. In addition, there were differential expressions of ube3a in different tissues of Japanese flounder, with the highest expression level observed in the fin, followed by the gills and skin (P ≤ 0.05). Subcellular localization analysis revealed that Ube3a is a cytoplasmic protein. We established an in vitro CSBV Infection model using Japanese flounder gill cell line (FG). After ube3a overexpression, the viral load was significantly lower than that of the control group (P ≤ 0.05). Contrastingly, after incubation of FG cells with an E3 ubiquitin ligase inhibitor, the viral load was significantly higher than in the control group (P ≤ 0.01). Then, the expression levels of NF-κB, traf3, and tnf-α after incubation with an E3 ubiquitin ligase inhibitor were examined. The results demonstrated that ube3a may exerted a significant Antiviral effect in Japanese flounder via the ubiquitination pathway.

Keywords

Inflammatory response; Japanese flounder; Ubiquitination; ube3a.

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