14-3-3 theta 蛋白, Human (GST)

14-3-3 theta protein, an adapter in diverse signaling pathways, recognizes phosphoserine or phosphothreonine motifs, modulating partner activity upon binding. It negatively regulates PDPK1 kinase activity, forms homodimers, and interacts with CDK16, phosphorylated RGS7, SSH1, phosphorylated CDKN1B, GAB2, phosphorylated PDPK1, and phosphorylated DAPK2. Additional interactions include PI4KB, TBC1D22A, TBC1D22B, SLITRK1, RIPOR2 isoform 2, INAVA, MARK2, MARK3, MARK4, and MEFV, highlighting its versatile role. 14-3-3 theta Protein, Human (GST) is the recombinant human-derived 14-3-3 theta protein, expressed by E. coli, with N-GST labeled tag. The total length of 14-3-3 theta Protein, Human (GST) is 245 a.a., with molecular weight of ~53 kDa.

14-3-3 theta 蛋白作为接头蛋白发挥作用，参与多种通用和专门信号通路的调节，通过识别磷酸丝氨酸或磷酸苏氨酸基序与众多伙伴相互作用。这种结合通常会导致相互作用伙伴的活性调节。值得注意的是，14-3-3 theta 负向调节 PDPK1 的激酶活性并形成同二聚体。它与 CDK16、磷酸化形式的 RGS7、SSH1、“Thr-198”磷酸化形式的 CDKN1B、GAB2、PDPK1 的“Ser-241”磷酸化形式以及 DAPK2 的“Thr-369”磷酸化形式相互作用。此外，14-3-3 theta 与 PI4KB、TBC1D22A、TBC1D22B、SLITRK1、RIPOR2 同工型 2、INAVA（模式识别受体刺激后增加）、MARK2、MARK3、MARK4 和 MEFV 相互作用。这些相互作用强调了 14-3-3 theta 在各种细胞过程和信号通路中的多功能作用。

Human

E. coli

N-GST

P27348 (M1-N245)

10971  [NCBI]

Approximately 53 kDa

Greater than 88% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.