AKT1 蛋白, Human (sf9, His)

AKT1 Protein, part of the AKT kinase family, crucially regulates metabolism, proliferation, survival, growth, and angiogenesis. Phosphorylating over 100 substrates, it orchestrates insulin-induced SLC2A4/GLUT4 translocation, impacting glucose uptake, and governs glycogen storage by inhibiting GSK3A/B. AKT1 mediates insulin-stimulated protein synthesis via mTORC1, regulates FOXO factors, influences NF-kappa-B-dependent gene transcription, and enhances CREB1 activity. It modulates lipid metabolism, inhibiting lipolysis, and is involved in cell migration, adhesion, placental development, and responses to growth factors like PDGF, EGF, insulin, and IGF-I. Its role in SPATA13-mediated regulation adds complexity, highlighting AKT1's central role in cellular homeostasis and responsiveness to cues. AKT1 Protein, Human (sf9, His) is the recombinant human-derived AKT1 protein, expressed by Sf9 insect cells, with C-His labeled tag. The total length of AKT1 Protein, Human (sf9, His) is 480 a.a., with molecular weight of ~57 kDa.

AKT1 蛋白与 AKT2 和 AKT3 一样是 AKT 激酶家族的成员，在调节代谢、增殖、细胞存活、生长和血管生成等多种细胞过程中发挥着关键作用。AKT1 通过众多下游底物的丝氨酸和/或苏氨酸磷酸化发挥作用，参与 100 多种报道的候选底物的调节，这证明了其多方面的性质。值得注意的是，它协调胰岛素诱导的 SLC2A4/GLUT4 葡萄糖转运蛋白易位，影响葡萄糖摄取。此外，AKT1 通过磷酸化 GSK3A 和 GSK3B 来控制葡萄糖以糖原形式储存，抑制其激酶活性。它参与多种途径，包括通过 mTORC1 信号途径介导胰岛素刺激的蛋白质合成以及调节 FOXO 因子，影响细胞定位。AKT1 还影响 NF-kappa-B 依赖性基因转录并正向调节 CREB1 活性，有助于促生存基因的转录。此外，AKT1 通过磷酸化 ATP 柠檬酸裂解酶 (ACLY) 并激活环核苷酸磷酸二酯酶 (PDE3B) 的 3B 同工型，从而在脂质代谢中发挥作用，从而降低环 AMP 水平并抑制脂肪分解。除了这些功能之外，AKT1 还参与细胞迁移、粘附组装和分解、胎盘发育以及对生长因子的多种细胞反应，包括血小板源性生长因子 (PDGF)、表皮生长因子 (EGF)、胰岛素和胰岛素样因子生长因子 I (IGF-I)。最近发现 AKT1 在 SPATA13 介导的细胞迁移和粘附调节中的作用增加了其功能的复杂性，突出了 AKT1 在细胞稳态和对环境信号的响应中的核心作用。

No Kinase Activity.

Human

Sf9 insect cells

C-His

P31749 (M1-A480)

207  [NCBI]

Approximately 57 kDa

Greater than 87% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 3 mM DTT, 0.5 mM PMSF, 5% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.