Aldolase B 蛋白, Human (GST)

Aldolase B protein serves a pivotal role in glycolysis by catalyzing the aldol cleavage of fructose 1,6-bisphosphate into dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate, as well as the reverse stereospecific aldol addition reaction in gluconeogenesis. Additionally, in fructolysis, it metabolizes fructose 1-phosphate, derived from the phosphorylation of dietary fructose by fructokinase, into dihydroxyacetone phosphate and D-glyceraldehyde. Beyond its enzymatic function, Aldolase B acts as an adapter and functions as a tumor suppressor by stabilizing a ternary complex with G6PD and TP53, inhibiting G6PD activity. This regulatory mechanism helps maintain control over oxidative pentose phosphate metabolism, contributing to the overall balance of cellular metabolic pathways. Aldolase B Protein, Human (GST) is the recombinant human-derived Aldolase B protein, expressed by E. coli , with N-GST labeled tag. The total length of Aldolase B Protein, Human (GST) is 363 a.a., with molecular weight of ~60 kDa.

醛缩酶 B 蛋白通过催化果糖 1,6-二磷酸羟醛裂解为磷酸二羟丙酮和 D-甘油醛 3-磷酸，以及糖异生中的反向立体特异性羟醛加成反应，在糖酵解中发挥关键作用。此外，在果糖分解过程中，它将膳食果糖通过果糖激酶磷酸化而产生的 1-磷酸果糖代谢为磷酸二羟丙酮和 D-甘油醛。除了其酶促功能外，醛缩酶 B 还充当接头，并通过稳定与 G6PD 和 TP53 的三元复合物来发挥肿瘤抑制因子的作用，从而抑制 G6PD 活性。这种调节机制有助于维持对氧化戊糖磷酸代谢的控制，有助于细胞代谢途径的整体平衡。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-GST

P05062 (A2-Y364)

229  [NCBI]

Approximately 60 KDa

Greater than 88% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.5.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.