Animal-Free BMP-13/GDF-6 蛋白, Human (His)

The BMP-13/GDF-6 protein is a key growth factor that regulates retinal protrusions, apoptosis, and dorsal-ventral positional information and is essential for the formation of the retinal tectum pattern. It is integral to the bones and joints of the limbs, skull, fingers and axial skeleton during skeletal development, shaping species-specific skeletal evolution. Animal-Free BMP-13/GDF-6 Protein, Human (His) is the recombinant human-derived animal-FreeBMP-13/GDF-6 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free BMP-13/GDF-6 Protein, Human (His) is 120 a.a., with molecular weight of ~14.50 kDa.

不含动物成分的 BMP-13/GDF-6 蛋白是一种关键的生长因子，控制着关键的过程，包括视网膜的增殖和细胞分化以及骨形成。它在视网膜发育过程中的细胞凋亡中的调节作用至关重要，有助于背腹位置信息的建立并控制视网膜顶盖图的形成。在骨骼发育的背景下，这种生长因子对于不同解剖区域（例如四肢、头骨、手指和中轴骨骼）的骨骼和关节的正常形成是不可或缺的。此外，它在描绘骨骼元素之间的边界方面发挥着关键作用，表明它在物种特异性骨骼进化中发挥着作用。在功能上，BMP-13/GDF-6 通过特定的生长因子受体亚基（包括 BMPR1A、BMPR1B、BMPR2 和 ACVR2A）对软骨形成组织分化进行正向调节，最终激活 SMAD1-SMAD5-SMAD8 复合物。值得注意的是，NOG 作为软骨形成分化调节的抑制剂。此外，BMP-13/GDF-6 参与诱导间充质干细胞的脂肪生成，与生长因子受体 BMPR1A、BMPR2 和 ACVR2A 结合，并通过同源二聚化机制激活 SMAD1-SMAD5-SMAD8 复合物和 MAPK14/p38具有二硫键连接的结构。

Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is 63-240 ng/mL.

Human

E. coli

C-His

Q6KF10 (T336-R455)

392255  [NCBI]

MTAFASRHGKRHGKKSRLRCSKKPLHVNFKELGWDDWIIAPLEYEAYHCEGVCDFPLRSHLEPTNHAIIQTLMNSMDPGSTPPSCCVPTKLTPISILYIDAGNNVVYKQYEDMVVESCGCR

Approximately 14.50 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 20 mM sodium citrate, 0.2 MNaCl, pH 3.5.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.