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  4. AQPZ 蛋白, E.coli (Cell-Free, His)

AQPZ 蛋白, E.coli (Cell-Free, His)

目录号: HY-P72013
产品使用指南

AQPZ 充当促进渗透驱动的水双向运动的通道。它在渗透压调节中发挥着至关重要的作用，积极促进细胞膨胀的维持，特别是在活跃生长的细胞体积快速膨胀期间。该通道能够使水快速进入或排出，对渗透压的突然变化迅速做出反应，凸显了其在细胞水稳态中的重要性。AQPZ 蛋白, E.coli (Cell-Free, His) 是重组的 AQPZ 蛋白，由 E. coli Cell-free 表达，带有 N-6*His 标签。AQPZ 蛋白, E.coli (Cell-Free, His) 全长 231 个氨基酸，分子量约为 ~27.7 kDa。

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描述

AQPZ serves as a channel facilitating osmotically driven water movement bidirectionally. It plays a crucial role in osmoregulation, actively contributing to the maintenance of cell turgor, particularly during rapid volume expansion in actively growing cells. This channel enables the swift entry or exit of water, responding promptly to sudden changes in osmolarity, highlighting its significance in cellular water homeostasis. AQPZ Protein, E.coli (Cell-Free, His) is the recombinant E. coli-derived AQPZ protein, expressed by E. coli Cell-free , with N-6*His labeled tag. The total length of AQPZ Protein, E.coli (Cell-Free, His) is 231 a.a., with molecular weight of ~27.7 kDa.
研究背景

AQPZ（水通道蛋白 Z）蛋白充当促进渗透驱动的水双向运动的通道。这种水通道蛋白在渗透压调节中起着至关重要的作用，并在快速生长的细胞中观察到的体积膨胀过程中参与维持细胞膨胀。AQPZ 能够快速进出水，对渗透压的突然变化迅速做出反应。该蛋白质介导双向水运输的能力突出了其在调节细胞水合作用和确保不同渗透条件下适当的细胞体积方面的重要性。
种属

E.coli
表达系统

E. coli Cell-free
标签

N-6*His
蛋白编号

P60844 (M1-D231)
基因 ID

58388674  [NCBI]
蛋白结构
N-term
6*His
AQPZ (M1-D231)
Accession # P60844
C-term
同用名
Bacterial nodulin-like intrinsic protein
氨基酸序列

MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFGLTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD

分子量

Approximately 27.7 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

AQPZ Protein, E.coli (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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