Arginase-1/ARG1 蛋白, Mouse (P.pastoris, His)

Arginase-1 (ARG1) plays a key role in the urea cycle, converting L-arginine into urea and L-ornithine. This cycle maintains L-arginine homeostasis, which is critical for nitric oxide synthase to compete for arginine. Arginase-1/ARG1 Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived Arginase-1/ARG1 protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of Arginase-1/ARG1 Protein, Mouse (P.pastoris, His) is 323 a.a., with molecular weight of ~36.8 kDa.

精氨酸酶-1 (ARG1) 是尿素循环的核心组成部分，对于 L-精氨酸转化为尿素和 L-鸟氨酸至关重要，后者是胶原蛋白合成和促进细胞增殖的生物能途径中至关重要的代谢物的前体。尿素循环主要在肝脏中活跃，其次在肾脏中活跃，在维持 L-精氨酸稳态方面发挥着关键作用，特别是在一氧化氮合酶 (NOS) 和精氨酸酶对细胞内精氨酸存在竞争关系的组织中。。除了其代谢功能之外，ARG1 还作为先天性和适应性免疫反应的关键调节因子，参与多形核粒细胞 (PMN) 的抗菌效应途径。PMN 细胞死亡后，ARG1 被释放，耗尽微环境中的精氨酸，抑制 T 细胞和自然杀伤 (NK) 细胞的增殖以及细胞因子的分泌。值得注意的是，在第 2 组先天淋巴细胞 (ILC2) 中，ARG1 会导致肺部急性 2 型炎症，影响 ILC2 的最佳增殖。此外，ARG1 在替代激活巨噬细胞或 M2 巨噬细胞的免疫反应中发挥多方面的作用，影响伤口愈合、组织再生、防御多细胞病原体和免疫抑制等过程，其结果因器官而异。在肿瘤浸润树突状细胞 (DC) 和骨髓源性抑制细胞 (MDSC) 中，ARG1 有助于抑制 T 细胞介导的抗肿瘤免疫，凸显其多样化且依赖于环境的免疫调节功能。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

P. pastoris

N-6*His

Q61176 (M1-K323)

11846  [NCBI]

MSSKPKSLEIIGAPFSKGQPRGGVEKGPAALRKAGLLEKLKETEYDVRDHGDLAFVDVPNDSSFQIVKNPRSVGKANEELAGVVAEVQKNGRVSVVLGGDHSLAVGSISGHARVHPDLCVIWVDAHTDINTPLTTSSGNLHGQPVSFLLKELKGKFPDVPGFSWVTPCISAKDIVYIGLRDVDPGEHYIIKTLGIKYFSMTEVDKLGIGKVMEETFSYLLGRKKRPIHLSFDVDGLDPAFTPATGTPVLGGLSYREGLYITEEIYKTGLLSGLDIMEVNPTLGKTAEEVKSTVNTAVALTLACFGTQREGNHKPGTDYLKPPK

Approximately 36.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.