ATP5D 蛋白, Human (His)

ATP5D Protein, a vital component of mitochondrial membrane ATP synthase (Complex V), facilitates ATP generation from ADP using the proton gradient established by respiratory chain electron transport complexes. As part of the F(1) domain and central stalk, ATP5D contributes to ATP hydrolysis through a rotary mechanism. The ATP synthase complex, involving ATP5D, is pivotal for cellular energy production via ATP synthesis. ATP5D Protein, Human (His) is the recombinant human-derived ATP5D protein, expressed by E. coli , with N-10*His labeled tag. The total length of ATP5D Protein, Human (His) is 146 a.a., with molecular weight of ~20 kDa.

ATP5D 蛋白是线粒体膜 ATP 合酶（也称为复合体 V）的重要组成部分。该酶负责利用穿过线粒体膜的质子梯度（由线粒体膜上的电子传递复合物建立的梯度）从 ADP 生成 ATP。呼吸链。ATP5D 所属的 F 型 ATP 酶由两个主要结构域组成：F(1)（容纳催化核心）和 F(0)（容纳膜质子通道）。在催化过程中，F(1) 催化结构域中的 ATP 周转通过涉及中央茎亚基的旋转机制与质子易位相关。ATP5D 是 F(1) 结构域和中央茎的一部分，有助于导致 ATP 水解的旋转。ATP5D 参与的 ATP 合酶复合体包含各种亚基，在通过 ATP 合成产生细胞能量中发挥着关键作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-10*His

P30049 (A23-E168)

513  [NCBI]

AEAAAAPAAASGPNQMSFTFASPTQVFFNGANVRQVDVPTLTGAFGILAAHVPTLQVLRPGLVVVHAEDGTTSKYFVSSGSIAVNADSSVQLLAEEAVTLDMLDLGAAKANLEKAQAELVGTADEATRAEIQIRIEANEALVKALE

Approximately 20 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of Tris-based buffer, 50% Glycerol or 50 mM Tris-HCL, 300 mM NaCL, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.