1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. AURC 蛋白, Human (Sf9, GST)

AURC 蛋白, Human (Sf9, GST)

目录号: HY-P701710
产品使用指南

AURC 是一种关键的丝氨酸/苏氨酸蛋白激酶,作为染色体过客复合物 (CPC) 的一部分,在有丝分裂中发挥着关键的调节作用,确保准确的染色体排列和分离。它有助于染色质诱导的微管稳定和纺锤体组装。AURC 蛋白, Human (Sf9, GST) 是重组的 AURC 蛋白,由 Sf9 insect cells 表达,带有 N-GST 标签。AURC 蛋白, Human (Sf9, GST) 全长 308 个氨基酸。

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描述

AURC is a key serine/threonine protein kinase that plays a critical regulatory role in mitosis as part of the chromosomal passenger complex (CPC), ensuring accurate chromosome alignment and segregation. It contributes to chromatin-induced microtubule stabilization and spindle assembly. AURC Protein, Human (Sf9, GST) is the recombinant human-derived AURC protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of AURC Protein, Human (Sf9, GST) is 308 a.a., .

研究背景

AURC 是一种丝氨酸/苏氨酸蛋白激酶,是染色体过客复合体 (CPC) 的关键组成部分,是有丝分裂的关键调节因子。CPC 复合物在着丝粒中是不可或缺的,可确保染色体准确排列和分离,这对于染色质诱导的微管稳定和纺锤体组装至关重要。除了在有丝分裂中发挥作用外,AURC 在减数分裂中也发挥作用,特别是在精子发生中。它与 AURKB 共享冗余细胞功能,并且可以挽救 AURKB 击倒。与 AURKB 类似,AURKC 在“Ser-10”和“Ser-28”处磷酸化组蛋白 H3。AURKC 还磷酸化 CPC 复合体亚基 BIRC5/survivin 和 INCENP,从而增强 AURKC 活性。此外,AURC 通过在“Ser-228”处磷酸化 TACC1(一种参与细胞分裂的蛋白质)来靶向它。

种属

Human

表达系统

Sf9 insect cells

标签

N-GST

蛋白编号

Q9UQB9 (S2-S309)

基因 ID

6795

蛋白结构
N-term
GST
AURC (S2-S309)
Accession # Q9UQB9
C-term
同用名
AURKC; Aurora kinase C; Aurora 3; Aurora/IPL1-related kinase 3; ARK-3; Aurora-related kinase 3; Aurora/IPL1/Eg2 protein 2; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase aurora-C
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

AURC Protein, Human (Sf9, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
AURC Protein, Human (Sf9, GST)
目录号:
HY-P701710
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