Bcl-2-like 蛋白 2 蛋白, Human (His)

Bcl-2-like protein 2 is a key regulator that promotes cell survival and prevents dexamethasone-induced apoptosis, which is critical for postmitotic Sertoli cells. It inhibits BAX, emphasizing its role in survival mechanisms. Bcl-2-like protein 2 Protein, Human (His) is the recombinant human-derived Bcl-2-like protein 2 protein, expressed by E. coli , with C-6*His labeled tag. The total length of Bcl-2-like protein 2 Protein, Human (His) is 171 a.a., with molecular weight of ~18.0 kDa.

Bcl-2 样蛋白 2 是细胞动力学中的关键参与者，通过促进细胞存活并有效阻止地塞米松诱导的细胞凋亡来发挥影响。这种多功能蛋白在有丝分裂后支持细胞的存活中发挥着关键作用，它抑制 BAX 的促死亡活性。复杂的分子网络通过其 C 末端结构域延伸至与 HIF3A 的相互作用，强调其参与复杂的信号传导途径。此外，Bcl-2 样蛋白 2 与 BOP 相互作用，进一步凸显了其在细胞稳态中的作用。Bcl-2 样蛋白 2 的细微功能强调了其在协调细胞生存机制中的重要性，并值得进一步探索以全面了解其在不同细胞环境中的分子贡献。

Measured by its binding ability in a functional ELISA. Immobilized BID at 5 μg/mL (100 μL/well) can bind human Bcl-2-like protein 2. The ED₅₀ for this effect is 10.09 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized BID at 5 μg/mL (100 μL/well) can bind human Bcl-2-like protein 2. The ED₅₀ for this effect is 10.09 ng/mL.

Human

E. coli

C-6*His

AAI13523.1 (A2-T172)

599  [NCBI]

ATPASAPDTRALVADFVGYKLRQKGYVCGAGPGEGPAADPLHQAMRAAGDEFETRFRRTFSDLAAQLHVTPGSAQQRFTQVSDELFQGGPNWGRLVAFFVFGAALCAESVNKEMEPLVGQVQEWMVAYLETRLADWIHSSGGWAEFTALYGDGALEEARRLREGNWASVRT

Approximately 18.0 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filter solution of 25 mM HEPES, 100 mM KCl, 20% Glycerol, pH 7.5 or 20 mM HEPES, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Liu HN, et al. MiR-93 Inhibits Trophoblast Cell Proliferation and Promotes Cell Apoptosis by Targeting BCL2L2 in Recurrent Spontaneous Abortion. Reprod Sci. 2020;27(1):152-162.  [Content Brief]

  [2]. Hartman ML, et al. BCL-w: apoptotic and non-apoptotic role in health and disease. Cell Death Dis. 2020;11(4):260. Published 2020 Apr 21.  [Content Brief]