BDNF 蛋白, Mouse (R129A, R130A, HEK293, C-His)

BDNF protein is an important signaling molecule that activates NTRK2 downstream cascades and affects multiple roles in neuronal development and function. It promotes survival and differentiation of the peripheral and central nervous system, affecting axonal and dendritic growth. BDNF Protein, Mouse (R129A, R130A, HEK293, C-His) is the recombinant mouse-derived BDNF protein, expressed by HEK293 , with C-6*His labeled tag and R129A, R130A, , , mutation. The total length of BDNF Protein, Mouse (R129A, R130A, HEK293, C-His) is 231 a.a., with molecular weight of ~35-42 kDa.

BDNF 蛋白作为关键信号分子，激活 NTRK2 下游的级联反应，并在神经元发育和功能中发挥多种作用。在发育过程中，BDNF 促进外周和中枢神经系统中特定神经元群的存活和分化，影响轴突生长、寻路以及树突生长和形态的调节。它是中枢神经系统各个区域突触传递和可塑性的主要调节因子，有助于适应性神经元反应，例如长时程增强 (LTP)、长期抑制 (LTD)、某些形式的短期突触可塑性、以及内在神经元兴奋性的稳态调节。这种多功能性强调了它在塑造神经元连接和功能方面的不可或缺的作用。此外，BDNF 通过 NGFR 和 SORCS2 形成的异二聚体受体激活信号级联，进一步影响突触可塑性和长期抑制。值得注意的是，它与 NGFR 和 SORCS2 的相互作用与促进神经元凋亡和生长锥塌陷有关，突出了它对神经元生理和发育的多方面影响。

Immobilized Recombinant Human BDNF at 1 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human TrkB. The ED₅₀ for this effect is ≤91.22 ng/mL.

Mouse

HEK293

C-6*His

P21237-1 (A19-R249, R129A, R130A)

12064

APMKEVNVHGQGNLAYPGVRTHGTLESVNGPRAGSRGLTTTSLADTFEHVIEELLDEDQKVRPNEENHKDADLYTSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVAAHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR

Approximately 33-42 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.