beta-Catenin 蛋白, Human (sf9, His-GST)

Beta-Catenin protein is an important component of the canonical Wnt pathway and regulates cellular processes. In the absence of Wnt, it complexes with AXIN1, AXIN2, APC, CSNK1A1, and GSK3B to promote CTNNB1 phosphorylation and degradation. beta-Catenin Protein, Human (sf9, His-GST) is the recombinant human-derived beta-Catenin protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of beta-Catenin Protein, Human (sf9, His-GST) is 781 a.a., with molecular weight of ~116 kDa.

Beta-Catenin 蛋白作为经典 Wnt 信号通路的关键下游成分，调节各种细胞过程。在没有 Wnt 的情况下，它与 AXIN1、AXIN2、APC、CSNK1A1 和 GSK3B 形成复合物，促进 CTNNB1 的磷酸化和泛素化以进行后续降解。Wnt 配体结合后，CTNNB1 在细胞核中积聚，作为 TCF/LEF 家族转录因子的共激活剂来激活 Wnt 响应基因。除了在 Wnt 信号传导中发挥作用外，β-连环蛋白还参与细胞粘附，作为 E-钙粘蛋白：连环蛋白粘附复合物的一部分。它在调节中心体凝聚力、CDK2/PTPN6/CTNNB1/CEACAM1介导的胰岛素内化中发挥作用，并促进神经发生。此外，β-连环蛋白与各种蛋白质相互作用，包括 AXIN1、SRC、TCF7L2 等，突显其参与控制多种细胞功能的复杂分子网络。β-连环蛋白的动态相互作用和双重作用强调了其在细胞稳态和信号级联中的重要性。

Human

Sf9 insect cells

N-His;N-GST

P35222 (M1-L781)

1499  [NCBI]

Approximately 116 kDa

Greater than 85% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, 25% glycerol, pH 8.0, 0.1 mM EDTA, 1 mM TCEP, 0.4 mM PMSF, 0.5 mM GSH.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.