1. 重组蛋白
  2. Enzymes & Regulators Ubiquitin Related Proteins
  3. Transferases (EC 2) Ubiquitin Enzymes
  4. E3 Ligases
  5. BRAP 蛋白, Human

BRAP 蛋白, Human

目录号: HY-P701535
产品使用指南

BRAP 蛋白充当 MAP 激酶激活的负调节因子,抑制 Raf/MEK 复合物的形成,并可能使 KSR1 支架蛋白失活。作为 Ras 响应性 E3 泛素连接酶,BRAP 在 Ras 激活后发生自多泛素化,释放其对 Raf/MEK 复合物形成的抑制作用。BRAP 蛋白, Human 是重组的 BRAP 蛋白,由 E. coli 表达,不带标签。BRAP 蛋白, Human 全长 591 个氨基酸。

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描述

BRAP protein acts as a negative regulator of MAP kinase activation, inhibits the formation of the Raf/MEK complex, and may inactivate the KSR1 scaffolding protein. As a Ras-responsive E3 ubiquitin ligase, BRAP undergoes autopolyubiquitination upon Ras activation, releasing its inhibitory effect on Raf/MEK complex formation. BRAP Protein, Human is the recombinant human-derived BRAP protein, expressed by E. coli , with tag free. The total length of BRAP Protein, Human is 591 a.a., .

研究背景

BRAP 蛋白作为 MAP 激酶激活的负调节因子,主要通过限制 Raf/MEK 复合物的形成,可能是通过 KSR1 支架蛋白的失活来实现。此外,它作为 Ras 响应性 E3 泛素连接酶发挥作用,在 Ras 激活后,会发生自动多泛素化,从而释放其对 Raf/MEK 复合物形成的抑制作用。除了在激酶信号传导中的作用之外,BRAP 还可以作为细胞质滞留蛋白,在核转运过程的调节中发挥作用。这种多方面的功能使 BRAP 成为调节关键细胞通路和反应的关键参与者。

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

Q7Z569 (S2-K592)

基因 ID

8315

蛋白结构
N-term
BRAP (S2-K592)
Accession # Q7Z569
C-term
同用名
BRAP; BRCA1-associated protein; BRAP2; Impedes mitogenic signal propagation; IMP; RING finger protein 52; RING-type E3 ubiquitin transferase BRAP2; Renal carcinoma antigen NY-REN-63
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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BRAP Protein, Human
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HY-P701535
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