Brk 蛋白, Mouse (sf9, His-GST)

Brk protein is a non-receptor tyrosine protein kinase that regulates signaling pathways critical for epithelial differentiation and tumor growth. Its context-dependent functions involve phosphorylating substrates such as RNA-binding proteins, transcription factors, and signaling molecules. Brk Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived Brk protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of Brk Protein, Mouse (sf9, His-GST) is 451 a.a., with molecular weight of ~66 kDa.

Brk 蛋白是一种非受体酪氨酸蛋白激酶，复杂地调节对正常上皮细胞的分化和维持以及肿瘤生长至关重要的多种信号通路。其功能似乎与环境相关，并且根据细胞类型和细胞内定位而变化。Brk 蛋白磷酸化各种底物，包括 RNA 结合蛋白（KHDRBS1/SAM68、KHDRBS2/SLM1、KHDRBS3/SLM2 和 SFPQ/PSF）、转录因子（STAT3 和 STAT5A/B）和信号分子（ARHGAP35/p190RhoGAP、PXN/桩蛋白、BTK/ATK、STAP2/BKS)。它还与信号通路中的上游蛋白相关，例如 ADAM15、EGFR、ERBB2、ERBB3 和 IRS4。在正常组织中，Brk 蛋白促进细胞分化和凋亡，而在肿瘤中，它通过使细胞对有丝分裂信号敏感、增强增殖、不依赖贴壁的存活和迁移/侵袭来促进癌症进展。它与 EGFR、ERBB2、ERBB3 的关联可能通过 BTK/AKT 和 PI3 激酶增强 EGF 诱导的信号传导，从而促进乳腺肿瘤的发生。Brk 蛋白通过促进 EGF 介导的 ARHGAP35/p190RhoGAP 磷酸化并激活 RAS，同时灭活 RhoA 来促进迁移和增殖。此外，Brk 蛋白还可激活 STAT3 和 STAT5B 以促进增殖。Brk 蛋白的核和细胞质定位发挥着不同的作用，核 Brk 蛋白可能调节正常上皮细胞的生长，而细胞质 Brk 蛋白激活致癌信号通路。

Mouse

Sf9 insect cells

N-His;N-GST

Q64434 (M1-V451)

20459  [NCBI]

MVSWDKAHLGPKYVGLWDFKARTDEELSFQAGDLLHVTKKEELWWWATLLDAEGKALAEGYVPHNYLAEKETVESEPWFFGCISRSEAMHRLQAEDNSKGAFLIRVSQKPGADYVLSVRDAQAVRHYRIWKNNEGRLHLNEAVSFSNLSELVDYHKTQSLSHGLQLSMPCWKHKTEPLPHWDDWERPREEFTLCKKLGAGYFGEVFEALWKGQVHVAVKVISRDNLLHQHTFQAEIQAMKKLRHKHILSLYAVATAGDPVYIITELMPKGNLLQLLRDSDEKALPILELVDFASQVAEGMCYLESQNYIHRDLAARNVLVTENNLCKVGDFGLARLVKEDIYLSHEHNVPYKWTAPEALSRGHYSIKSDVWSFGVLLHEIFSRGQMPYPGMSNHETFLRVDAGYRMPCPLECPPNIHKLMLSCWSRDPKQRPCFKDLCEKLTGITRYENLV

Approximately 66 kDa

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.