2. 重组蛋白
  3. Enzymes & Regulators Biotinylated Proteins
  4. Protein Tyrosine Kinases
  5. Brutons Tyrosine Kinase (BTK)
  6. BTK 蛋白, Human (Biotinylated, sf9, His, Avi)

BTK 蛋白, Human (Biotinylated, sf9, His, Avi)

目录号: HY-P702730
COA 产品使用指南

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重组蛋白定制服务

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2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
10 μg ￥3500 In-stock
50 μg ￥9800 In-stock
100 μg   询价  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

生物活性

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. The Km of BTK for its substrate EGFRtide is 137 μM.
种属

Human
表达系统

Sf9 insect cells
标签

His;Avi
蛋白编号

Q06187-1 (A2-S659, C481S)
基因 ID

695
同用名
BTK; Bruton agammaglobulinemia tyrosine kinase; tyrosine-protein kinase BTK;
分子量

80.4 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
性状

溶液.
组分

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCl, 150 mM NaCl, 10% glycerol, 1 mM DTT, pH 7.5.

内毒素含量

<1 EU/μg, determined by LAL method.
复溶方法

Please use rapid thawing with running water to thaw the protein.
保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料

COA (148 KB)

产品使用指南 (1213 KB)

BTK Protein, Human (Biotinylated, sf9, His, Avi) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称：
BTK Protein, Human (Biotinylated, sf9, His, Avi)
目录号：
HY-P702730
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