Calcineurin A/PPP3CA 蛋白, Human (sf9, His)

Calcineurin A, also known as PPP3CA, is a calcium-dependent, calmodulin-stimulated protein phosphatase that plays a pivotal role in transducing intracellular Ca(2+)-mediated signals. Known substrates often contain a PxIxIT motif and/or an LxVP motif, highlighting the specificity of its phosphatase activity. In response to elevated Ca(2+) levels, Calcineurin A dephosphorylates and activates phosphatase SSH1, leading to cofilin dephosphorylation. Furthermore, upon increased Ca(2+) levels following mitochondrial depolarization, Calcineurin A dephosphorylates DNM1L, inducing its translocation to the mitochondrion. The protein also positively regulates CACNA1B/CAV2.2-mediated Ca(2+) release probability at hippocampal neuronal soma and synaptic terminals. Calcineurin A dephosphorylates and activates transcription factor NFATC1, thereby regulating NFAT-mediated transcription by promoting nuclear translocation. Its broad array of functions includes the dephosphorylation of heat shock protein HSPB1, transcription factor ELK1, DARPP32, CRTC2, TFEB, and KLHL3, suggesting a crucial role in various cellular processes such as kidney development, secretion of salivary enzymes, bone formation, immune responses, and regulation of ion transport. The multifaceted roles of Calcineurin A underscore its significance in diverse cellular functions and signal transduction pathways. Calcineurin A/PPP3CA Protein, Human (sf9, His) is the recombinant human-derived Calcineurin A/PPP3CA protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of Calcineurin A/PPP3CA Protein, Human (sf9, His) is 520 a.a., with molecular weight of ~60 kDa.

钙调磷酸酶 A，也称为 PPP3CA，是一种钙依赖性、钙调蛋白刺激的蛋白磷酸酶，在转导细胞内 Ca(2+) 介导的信号中发挥关键作用。已知的底物通常含有 PxIxIT 基序和/或 LxVP 基序，突出了其磷酸酶活性的特异性。为了响应 Ca(2+) 水平升高，钙调神经磷酸酶 A 去磷酸化并激活磷酸酶 SSH1，从而导致肌动蛋白丝切蛋白去磷酸化。此外，线粒体去极化后 Ca(2+) 水平增加，钙调神经磷酸酶 A 使 DNM1L 去磷酸化，诱导其易位至线粒体。该蛋白还正向调节海马神经元胞体和突触末梢 CACNA1B/CAV2.2 介导的 Ca(2+) 释放概率。钙调神经磷酸酶 A 去磷酸化并激活转录因子 NFATC1，从而通过促进核转位来调节 NFAT 介导的转录。其广泛的功能包括热休克蛋白 HSPB1、转录因子 ELK1、DARPP32、CRTC2、TFEB 和 KLHL3 的去磷酸化，表明在肾脏发育、唾液酶分泌、骨形成、免疫反应等各种细胞过程中发挥着至关重要的作用和离子传输的调节。钙调神经磷酸酶 A 的多方面作用强调了其在多种细胞功能和信号转导途径中的重要性。

Human

Sf9 insect cells

N-His

Q08209 (S2-Q521)

5530  [NCBI]

Approximately 60 kDa

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.