Carboxylesterase 1C 蛋白, Mouse (P.pastoris, His)

The carboxylesterase 1C protein is essential for xenobiotic detoxification and ester/amide prodrug activation, as well as participating in extracellular metabolism. Its multifunctional effects include processing of pulmonary surfactant. Carboxylesterase 1C Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived Carboxylesterase 1C protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of Carboxylesterase 1C Protein, Mouse (P.pastoris, His) is 532 a.a., with molecular weight of ~60.6 kDa.

羧酸酯酶 1C 蛋白在异生物质的解毒以及酯和酰胺前药的激活中发挥着至关重要的作用。这种多功能酶积极参与细胞外代谢，特别有助于肺表面活性剂的处理。它参与外源代谢和前药激活，强调了它在细胞防御机制和治疗干预中的重要性。羧酸酯酶 1C 的双重功能突出了其调节药物生物利用度和促进外来物质分解的能力，说明了其在维持细胞稳态和药物代谢中的重要作用。

The enzyme activity is measured by its ability to cleave substrate 4-NPA, The Km is 1.198 - 2.303 mM.

Mouse

P. pastoris

N-6*His

P23953 (H19-H550)

13884  [NCBI]

HSLLPPVVDTTQGKVLGKYISLEGFEQPVAVFLGVPFAKPPLGSLRFAPPQPAEPWSFVKNATSYPPMCSQDAGWAKILSDMFSTEKEILPLKISEDCLYLNIYSPADLTKSSQLPVMVWIHGGGLVIGGASPYNGLALSAHENVVVVTIQYRLGIWGLFSTGDEHSPGNWAHLDQLAALRWVQDNIANFGGNPDSVTIFGESSGGISVSVLVLSPLGKDLFHRAISESGVVINTNVGKKNIQAVNEIIATLSQCNDTSSAAMVQCLRQKTESELLEISGKLVQYNISLSTMIDGVVLPKAPEEILAEKSFNTVPYIVGFNKQEFGWIIPMMLQNLLPEGKMNEETASLLLRRFHSELNISESMIPAVIEQYLRGVDDPAKKSELILDMFGDIFFGIPAVLLSRSLRDAGVSTYMYEFRYRPSFVSDKRPQTVEGDHGDEIFFVFGAPLLKEGASEEETNLSKMVMKFWANFARNGNPNGEGLPHWPEYDEQEGYLQIGATTQQAQRLKAEEVAFWTELLAKNPPETDPTEH

Approximately 60.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.