1. 重组蛋白
  2. Cytokines and Growth Factors Receptor Proteins
  3. Chemokine & Receptors G-Protein-Coupled Receptors (GPCRs)
  4. CC Chemokine Receptor Chemokine Receptor
  5. CCR5
  6. CCR5 蛋白, Human (Cell-Free, His)

CCR5 蛋白, Human (Cell-Free, His)

目录号: HY-P702233
产品使用指南

CCR5 蛋白是炎症性 CC 趋化因子(如 CCL3/MIP-1-α、CCL4/MIP-1-β 和 RANTES)的受体,可传导信号,提高细胞内钙水平。它调节粒细胞谱系并促进 T 淋巴细胞迁移至感染部位。在微生物感染中,CCR5CD4 一起充当 HIV-1 的辅助受体,强调了其在细胞对感染的反应中的作用。CCR5 蛋白, Human (Cell-Free, His) 是重组的 CCR5 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。CCR5 蛋白, Human (Cell-Free, His) 全长 352 个氨基酸,分子量为 43.9 kDa。

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描述

CCR5 Protein, a receptor for inflammatory CC-chemokines like CCL3/MIP-1-alpha, CCL4/MIP-1-beta, and RANTES, transduces signals, elevating intracellular calcium levels. It regulates granulocytic lineage and facilitates T-lymphocyte migration to infection sites. In microbial infection, CCR5 acts as a coreceptor, along with CD4, for HIV-1, emphasizing its role in the cellular response to infections. CCR5 Protein, Human (Cell-Free, His) is the recombinant human-derived CCR5 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of CCR5 Protein, Human (Cell-Free, His) is 352 a.a., with molecular weight of 43.9 kDa.

研究背景

CCR5 作为多种炎症性 CC 趋化因子(包括 CCL3/MIP-1-alpha、CCL4/MIP-1-beta 和 RANTES)的受体发挥作用,导致信号转导,从而提高细胞内钙离子水平。该受体参与粒细胞谱系增殖或分化的调节,并作为趋化受体在促进 T 淋巴细胞迁移至感染部位方面发挥着至关重要的作用。在微生物感染的情况下,CCR5 充当人类免疫缺陷病毒-1/HIV-1 的辅助受体,CD4 是主要受体,强调了其在细胞对感染反应中的重要性。

种属

Human

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

P51681 (M1-L352)

基因 ID

1234

蛋白结构
N-term
10*His
CCR5 (M1-L352)
Accession # P51681
C-term
同用名
C-C chemokine receptor type 5; CHEMR13; HIV-1 fusion coreceptor
氨基酸序列

MDYQVSSPIYDINYYTSEPCQKINVKQIAARLLPPLYSLVFIFGFVGNMLVILILINCKRLKSMTDIYLLNLAISDLFFLLTVPFWAHYAAAQWDFGNTMCQLLTGLYFIGFFSGIFFIILLTIDRYLAVVHAVFALKARTVTFGVVTSVITWVVAVFASLPGIIFTRSQKEGLHYTCSSHFPYSQYQFWKNFQTLKIVILGLVLPLLVMVICYSGILKTLLRCRNEKKRHRAVRLIFTIMIVYFLFWAPYNIVLLLNTFQEFFGLNNCSSSNRLDQAMQVTETLGMTHCCINPIIYAFVGEKFRNYLLVFFQKHIAKRFCKCCSIFQQEAPERASSVYTRSTGEQEISVGL

分子量

43.9 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CCR5 Protein, Human (Cell-Free, His)
目录号:
HY-P702233
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