Semaphorin-4D/SEMA4D 蛋白, Human (713a.a, HEK293, His)

CD100 Protein, Human (HEK293, His) is a protein from the semaphorin family with important roles in the vascular, nervous and immune systems.

Semaphorin 4D、Sema4D 或 CD100 属于 4 类信号蛋白，是所谓的“免疫信号蛋白”之一。CD100 由大多数造血细胞（包括 B 和 T 淋巴细胞、自然杀伤细胞和骨髓细胞以及内皮细胞）产生，根据细胞类型和生物体，通过与不同受体结合来发挥作用。除了在膜中表达外，CD100 还以可溶形式 (sCD100) 存在，它是通过蛋白水解裂解以激活依赖性方式从膜 CD100 (mCD100) 产生的^[1]。

Human

HEK293

C-6*His

Q92854 (M22-R734)

10507  [NCBI]

MAFAPIPRITWEHREVHLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVFAVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSATSLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSPDSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKWTSFLKARLICSRPDSGLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVGLSAVCAYNLSTAEEVFSHGKYMQSTTVEQSHTKWVRYNGPVPKPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYTQIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFEPVQTLLLSSKKGNRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCAWSPPTATCVALHQTESPSRGLIQEMSGDASVCPDKSKGSYRQHFFKHGGTAELKCSQKSNLARVFWKFQNGVLKAESPKYGLMGRKNLLIFNLSEGDSGVYQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATKVLVASTQGSSPPTPAVQATSSGAITLPPKPAPTGTSCEPKIVINTVPQLHSEKTMYLKSSDNRHHHHHH

Approximately 112 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Maria C A Luque, et al. CD100 Effects in Macrophages and Its Roles in Atherosclerosis. Front Cardiovasc Med. 2018 Sep 28;5:136.  [Content Brief]